黄柏饮片标准汤剂制备及质量标准研究  

Preparation and quality standard of standard decoction of Phellodendri Chinensis Cortex pieces

作　　者：王淑慧 邓哲 章军[1] 焦梦姣[1] 张国媛 石佳 程锦堂 刘安 WANG Shu-hui1 , DENG Zhe1,2, ZHANG Jun1 , JIAO Meng-jiao1 , ZHANG Guo-yuan1 , SHI Jia1 , CHENG Jin-tang1 , LIU An1(1. Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; 2. Jiangxi University of Traditional Chinese Medicine, Nanehang 330004, Chin)

机构地区：[1]中国中医科学院中药研究所中药鉴定与安全性检测评估重点实验室,北京100700 [2]江西中医药大学,江西南昌330004

出　　处：《中国中药杂志》2018年第5期873-878,共6页China Journal of Chinese Materia Medica

基　　金：国家中药标准项目(ZYBZH-Y-JY-27);中国中医科学院“十三五”重点领域研究专项(ZZ10-007)

摘　　要：制备黄柏饮片标准汤剂并建立质量标准，为黄柏配方颗粒质量评价提供标准参照。从市场上收集15批不同质量的黄柏饮片，按照中药饮片标准汤剂工艺规范制备黄柏饮片标准汤剂，计算指标成分小檗碱和黄柏碱的转移率，测定于膏得率、pH等参数；建立指标成分含量测定和指纹图谱方法。结果表明黄柏饮片标准汤剂中小檗碱和黄柏碱转移率范围分别为36．4％～56．6％，47．5％～83．3％；平均转移率分别为48．9％，63．1％。干膏得率为12：81％～19．41％，平均干膏得率为16．54％；pH为5．2～5．9。15批黄柏饮片标准汤剂指纹图谱与对照指纹图谱相似度均〉0．9。该研究建立的制备方法稳定、质量标准完善，可用于黄柏配方颗粒质量评价。To prepare standard decoction of Phellodendri Chinensis Cortex pieces, establish quality standards, and provide refer- ence for evaluating the quality of formula granule of Phellodendri Chinensis Cortex. 15 batches Phellodendri Chinensis Cortex pieces in different levels of quality were collected from market. The standard extraction process was used to prepare the standard decoction, and then the transfer rate of the index components berberine and phellodendrine was calculated to measure dry extraction ratio and pH val- ue. So the method for Phellodendri Chinensis Cortex＇s fingerprint and content determination of index components was established. The result revealed that the extraction ratio was from 12.81% to 19.41%, with an average value of 16.54%. The transfer rate of berberine was at the range of 36.4%-56.6% , with an average value of 48.9%. The transfer rate of Phellodendri Chinensis Cortex piece was at the range of 47.5% -83.3% , with an average value of 63.1%. The pH value was between 5.2 and 5.9. The Similarity Evaluation System for Chromatographic Fingerprint of traditional Chinese medicine TCM （2012A） was used to analyze and compare the fingerprint and the similarity between the fingerprint of formula granule of Phellodendri Chinensis Cortex and the reference fingerprint was over O. 9. The preparation method in this paper showed good precision, stability and repeatability in fingerprint analysis, suitable for quality evaluation of Phellodendri Chinensis Cortex pieces standard decoction.

关 键 词：黄柏饮片 标准汤剂 质量标准 

分 类 号：R284.1[医药卫生—中药学]