蛋白质组学技术及其在中药作用机制研究中的应用  

Proteomics and its application to determine mechanism of action of traditional Chinese medicine

作　　者：辛萍[1] 匡海学[1] 李晓亮[1] 王宇[1] 张本梅 布赫 王知斌[1] 孟永海[1] 王艳宏[1] 王秋红[2] XIN Ping1, KUANG Hai-xue1 , LI Xiao-liang1 , WANG Yu1 , ZHANG Ben-mei1 , BU He1, WANG Zhi-bin1 , MENG Yong-hai1 , WANG Yan-hongI, WANG Qiu-hong2.(1. Key Laboratory of Chinese Materia Medica, Ministry of Education, Heilongfiang Key Laboratory of Traditional Chinese Medicine Pharmacodynamic Material, Heilongjiang University of Chinese Medicine, Harbin 150040, China 2. Guangclong Pharmaceutical University, Guangzhou 510006, Chin)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室黑龙江省中药及天然药物药效物质基础研究重点实验室,黑龙江哈尔滨150040 [2]广东药科大学,广东广州510006

出　　处：《中国中药杂志》2018年第5期904-912,共9页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2013CB531801)

摘　　要：历经千年的传承，中药的有效性、实用性和科学性已毋庸置疑，但是其多成分、多靶点、多层次的作用特点给作用机制的研究带来困难，阻碍了中药的现代化和国际化进程。蛋白质组学技术是从整体的角度分析细胞内动态变化的蛋白质的组成与活动规律，与中药的“整体观”和“动态观”不谋而合，为阐明中药作用机制带来了曙光。近年，蛋白质组学技术在中药作用机制研究中的应用取得了实质性的进展，该文系统的介绍了蛋白质组学的核心技术，并着重综述了近几年蛋白质组学在中药复方、单味中药和中药单体化合物作用机制研究中的应用，以期为中药作用机制的研究提供新的思路和方法。There is no doubt that the traditional Chinese medicine（TCM） is effective, practical and scientific after it was used for thousands of years. However, the mechanisms of action of many TCM are still unclear because of their multi-component, multi-target and multi-level features, which hinder the modernization and internationalization of the TCM. Proteomics is to analyze the composition and activity of intracellular proteins which are changing dynamically from a holistic perspective. It is consistent with the holistic and dy- namic views of the TCM and brings about the hope of clarifying the mechanism of action of the TCM. In recent years, great progress has been made in the application of proteomics to determine the mechanism of the TCM. This article introduced the core technologies of proteomics and systematically summarized the applications of proteomics in the study of the mechanism of the Chinese medicinal formu- lae. sinzle Chinese medicine and monomeric compounds from the TCM to provide innovative ideas and methods for reference.

关 键 词：蛋白质组学 中药 作用机制 

分 类 号：R285[医药卫生—中药学]