柑橘PGIP的B细胞抗原表位分析和原核表达  

B Cell Epitopes Analysis and Prokaryotic Expression of PGIP in Citrus

作　　者：陈波 罗庆华[1] 谭雅芹 闫慧清 CHEN Bo, LUO Qing-hua, TAN Ya-qin, YAN Hui-qing(College of Life Science, Guizhou Normal University, Guiyang 550001, Chin)

机构地区：[1]贵州师范大学生命科学学院,贵州贵阳550001

出　　处：《现代食品科技》2018年第4期18-22,29,共6页Modern Food Science and Technology

基　　金：国家自然科学基金资助项目(31660554);博士科研启动项目(11904-0514156;11904-0514157);"通识教育基础课程-营养与健康"课程团队项目和品牌团队(KT2016015B)

摘　　要：柑橘是重要的经济园艺植物,但是部分人群在食用柑橘后会造成过敏现象,其中多聚半乳糖醛酸酶抑制蛋白(PGIP)是柑橘中重要的过敏原。本文利用Jameson-Wolf法、Kyte-Doolittle法、Emini法和Karplus-Schulz法对柑橘过敏原蛋白PGIP的抗原指数、亲水性、蛋白表面可及性及柔韧性进行分析,通过构建PET-28 a-PGIP载体转移到大肠杆菌(Escherichia coli)中进行原核表达。结果表明:当PGIP二级结构是β-转角和无规则卷曲,同时氨基酸序列的抗原指数>0、柔韧性>0、亲水性>0和蛋白表面可及性>1时的氨基酸时,过敏原蛋白PGIP的B细胞表位结合氨基酸的序列区域是在19~22、37~41、49~52、68~69、117~120、163~164、173~177、221~226、236~240。通过分析氨基酸的区域及原核表达,为寻找柑橘过敏原蛋白PGIP的B细胞抗原表位的最佳优势区域提供支持,同时也为消除柑橘过敏原蛋白PGIP对人体的影响的进一步研究提供理论基础。Citrus is an important horticulture. However consumption of citrus fruits could cause allergen for some people. With the method of Jameson-Wolf, Kyte-Doolittle, Emini and Karplus-Schulz, the antigenicity, hydrophilicity, protein surface accessibility and flexibility of citrus allergen protein PGIP（polygalacturonase inhibitor protein） were predicted. Meanwhile PET-28 a-PGIP vector was constructed, and then transfer to Escherichia coli. The target gene was induced and expressed. The results showed： When the secondary structure of amino acid was β-angle and irregular curl, the antigen index0, the flexibility0, the hydrophilicity0, the protein surface accessibility1 amino acid region, the sequences of the B cell epitope binding amino acid were 19-22, 37-41, 49-52, 68-69, 117-120, 163-164, 173-177, 221-226, 236-240. By estimating the amino acid region, it provides support for finding the best possible region of the B cell epitope of citrus allergen protein PGIP, and also provides a theoretical basis for the further study of citrus allergen protein PGIP.

关 键 词：柑橘 多聚半乳糖醛酸酶抑制蛋白 B细胞表位 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]