小麦-长穗偃麦草T7BS·7EL易位系鉴定及7EL小片段易位诱导  

Identification of Wheat-Thinopyrum elongatum Translocation Line T7BS·7EL and Induction of Small Fragment Translocations Involving 7EL Chromosome

作　　者：李海凤[1,2] 罗贤磊 段亚梅 戴毅[1] 高勇[1] 张军[2] 张璐璐[1] 陈建民[1] LI Haifeng;LUO Xianlei;DUAN Yamei;DAI Yi;GAO Yong;ZHANG Jun;ZHANG Lulu;CHEN Jianmin(College of Bioscience and Biotechnology,Yangzhou University,Yangzhou,Jiangsu 225009,China;Yangzhou Polytechnic College,Yangzhou,Jiangsu 225009,China)

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009 [2]扬州市职业大学,江苏扬州225012

出　　处：《麦类作物学报》2018年第5期513-520,共8页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(31071406);国家转基因生物新品种重大专项(2014ZX0800205B);江苏省农业生物学重点实验室项目(49114042015K003);农业与农产品安全国际合作项目

摘　　要：为了有效利用含二倍体长穗偃麦草7E染色体的种质材料,利用荧光原位杂交和分子标记技术以及赤霉病抗性评价,对从中国春-二倍体长穗偃麦草7E代换系DS7E(7B)与扬麦16杂交的F2种子辐射后代中选育的小麦-长穗偃麦草易位系TW-7EL2进行了鉴定。荧光原位杂交(FISH)结果表明,易位染色体的小麦染色体片段与小麦7B短臂的杂交信号相似;分子标记检测结果表明,在易位系中能够扩增出7EL特异条带,但缺失小麦7BL特异条带。综合上述结果,将该易位系命名为T7BS·7EL。且其赤霉病抗性显著高于中国春和扬麦16,与苏麦3号接近,是小麦赤霉病抗病育种的新种质。继续利用1 200 rad^(60)Co-γ射线辐射处理易位系T7BS·7EL的成熟花粉并授予扬麦158,从其纯合易位系的辐射后代M_1中检测到长穗偃麦草7EL染色体的小片段中间插入易位、顶端易位和7EL染色体缺失等结构变异的单株15株,诱变频率为15.62%,表明利用小麦-长穗偃麦草整臂易位系进行成熟花粉辐射,可以高效诱导产生小片段易位材料。In order to effectively utilize the germplasm material with diploid Thinopyrum elongatum chromosome 7E,the translocation line TW-7EL2 was identified from the plant progenies of irradiated F2 seeds from the cross between Yangmai 16 and Chinese Spring- Th.elongatum substitution line DS7E（7B） through chromosome FISH,molecular marker analysis and FHB resistant test. The FISH analysis showed that the hybridization signals of wheat chromosome segment of the translocation chromosome in TW-7EL2 was similar to that of wheat chromosome 7BS.The marker Xgwm333 located on chromosome 7BL amplified Th.elongatum chromosome 7EL specific band in TW-7EL2 and DS7E（7B）,but the specific band of wheat chromosome 7BL was absent. The results of FISH and molecular marker analysis showed the translocation line was designated as T7BS·7EL.The resistance level of the translocation line was higher than that of Chinese Spring and Yangmai 16 and similar to that of the resistant check Sumai 3 after a successive three-year field FHB resistant test. The result showed the translocation line T7BS·7EL with the stable FHB resistance and feasibility can be used as a new germplasm for improving FHB resistance in wheat. On the basis of the above studies,the mature pollen of homozygous translocation line T7BS·7EL were irradiated with 1 200 rad 60 Co-γ ray before flowering,and then pollinated to common wheat variety Yangmai 158. Among the 96 M 1 plants,15 chromosome aberration plants involving 7EL were screened by GISH,including interstitial translocation chromosomes,terminal translocation chromosomes and 7EL deletion chromosomes,and the mutation frequency was 15.62%. These results showed that wheat-Th.elongatum whole arm translocation line of mature pollen irradiation could efficiently induce small segment translocations.

关 键 词：小麦 长穗偃麦草 易位系 赤霉病抗性 

分 类 号：S512.1[农业科学—作物学] S330