邻香草醛缩壳寡糖席夫碱及其Cu(Ⅱ)配合物与DNA的相互作用  

Interaction of Schiff Base Derived from Oligochitosan and o-Vanillin and Its Cu(Ⅱ)Complex with DNA

作　　者：冯小强 李小芳 朱元成 FENG Xiaoqiang;LI Xiaofang;ZHU Yuancheng(School of Chemical Engineering and Technogogy, Tianshui Normal University, Tianshui 741001 , Gansu Province, China)

机构地区：[1]天水师范学院化学工程与技术学院,甘肃天水741001

出　　处：《吉林大学学报（理学版）》2018年第3期732-737,共6页Journal of Jilin University:Science Edition

基　　金：甘肃省青年科技基金(批准号:1606RJYE247);甘肃省高等学校科研项目(批准号:2016B-071);天水市科技支撑项目(批准号:2016);天水师范学院中青年教师科研项目(批准号:TSA1508)

摘　　要：用邻香草醛对天然高分子壳寡糖进行改性,将其与具有优异配位性能的席夫碱和Cu2+配位,制备邻香草醛席夫碱改性壳寡糖的铜配合物,并通过循环伏安法、紫外光谱、黏度和熔点法,考察席夫碱及铜配合物与DNA的作用机制.结果表明:配合物具有电化学活性,当扫描速率为0.025~1.0V/s时,配合物在玻碳电极上的反应主要由吸附过程控制;加入DNA后,配合物的氧化峰电流减小,峰电位微弱移动,VCOS-Cu13和VCOS-Cu31与DNA可形成1∶1(物质的量比)型配合物,结合常数分别为0.78×10~4,0.69×10~4 L/mol;配合物的吸收峰强度降低,峰位出现红移;DNA的相对黏度和熔点在加入配合物后增大;席夫碱及两种配合物以嵌插模式与DNA作用.The natural polymer oligochitosan was modified using o-vanillin.By the excellent coordination properties of the Schiff base structure,the copper complex of o-vanillic aldehyde modified oligochitosan was prepared.Cyclic voltammetry curves indicated that Schiff base-copper complexes possessed electrochemical activity,and the reaction of central Cu^2＋at glassy carbonelectrodes was controlled by diffusion process mechanism in the range of scan rate from 0.025—1.0 V/s. We investigated the mechanism of the interaction of Schiff base and Cu complex with DNA by using cyclic voltammetry,ultraviolet spectrum,viscosity and melting point method.The results show that,after adding DNA,the oxidation peak current of the complex decreases and the peak potential moves weakly.VCOS-Cu13,VCOS-Cu31 and DNA can form complexes of molar ratio of 1∶1,and the binding constants are 0.78×10^4,0.69×10^4 L/mol.The intensities of absorption peaks of complexes are reduced and the peak position appears red shift.The relative viscosity and melting point of DNA increase after adding the complex.There are interca lation action between different molar of complexand DNA.

关 键 词：席夫碱 Cu(Ⅱ)配合物 结合DNA 

分 类 号：O611.4[理学—无机化学]