孕牛血清中早孕因子的分离纯化与鉴定  

Isolation and Purification of Early Pregnancy Factor from Pregnant Bovine Serum by Using Chromatography Techniques

作　　者：王晓姗 陈树林[1] 张文华[1] 张宏刚[1] WANG Xiao-shan;CHEN Shu-lin;ZHANG Wen-hua;ZHAN Hong-gang(College of Veterinary Medicine,Northwest A＆F University,Yangling Shaanxi 712100)

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《中国牛业科学》2018年第2期22-24,共3页China Cattle Science

摘　　要：为了从妊娠4~5个月健康孕牛血清中分离得到纯化的早孕因子(EPF),通过DEAE-Sepharos F.F离子交换层析,CM–Sepharose F.F离子交换层析,Con A-Sepharose 4B亲和层析和Heparin Sepharose Cl 6B亲和层析分离纯化技术,从孕牛血清中分离出纯化出EPF。采用玫瑰花环抑制实验(Ea-RIT)对各阶段产物EPF活性进行检测,最终纯化出具有早孕因子活性的HE-2成分。将HE-2经SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)出现分子量为53.6KU蛋白质条带。该方法得到分离纯化的EPF,为进一步研制早孕因子单克隆抗体的制备做准备。To get the pure early pregnancy factor（EPF）from the pregnant bovine serum of 4～5 months,we used a series of techniques such as DEAE-Sepharose,CM-Sepharose ion-exchange chromatography,Con A-Sepharose 4 Baffinity chromatography and Heparin Sepharose Cl 6 Baffinity chromatography,.EPF activity of each fraction was determined by the rosette inhibition test.The result showed that the fraction H-2 has EPF activity.The SDS-PAGE showed molecular weights of 53.6 kD protein.This method has been purified EPF for the preparation of monoclonal antibodies.

关 键 词：孕牛血清 早孕因子 纯化 分子量 

分 类 号：S823[农业科学—畜牧学]