2个番木瓜品种组培苗成苗率研究  

Study on the Survival Rate of Two Varieties of Papaya Plantlets for Tissue Culture Seedling

作　　者：谢智旭 沈文涛[2] 言普[2] 庹德财[2] 黎小瑛[2] 周鹏 XIE Zhi-xu;SHEN Wen-tao;YAN Pu(Institute of Tropical Agriculture and Forestry,Hainan University,Haikou, Hainan570228;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou, Hainan 57110)

机构地区：[1]海南大学热带农林学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《安徽农业科学》2018年第14期47-50,85,共5页Journal of Anhui Agricultural Sciences

基　　金：中国热带农业科学院热带生物技术研究所基本科研业务费专项(1630052016005)

摘　　要：[目的]寻找一种针对番木瓜成苗率高、商品苗质量优的育苗技术。[方法]选用番木瓜品种"梭罗"作为试验材料,MS为基本培养基,添加IBA、NAA、KT等生长调节剂,利用正交试验进行生长调节剂不同配比生根培养基组合筛选。[结果]最佳生根调节剂组合:IBA 0.75 mg/L,NAA 0.05 mg/L,KT 0.01 mg/L,其生根率为66.7%,但自育品种"蜜红"并未获得同样的效果。最佳生根水浓度为50 mg/L,其成苗率最高达86.1%。在不考虑时间成本情况下,生根水浸泡时间8 h,成苗率最高达87.5%。[结论]2个番木瓜品种成苗率基本一致。[Objective]To find a breeding technique for papaya with high seedling rate and good quality commercial seedling. [Method]‘suoluo＇variety was selected as the experimental material,and MS was used as the basic culture medium. Growth regulator combinations that contained IBA,NAA,KT was designed,and the orthogonal experiment was used with the different ratio of growth regulator for rooting medium screening. [Result]The optimum combining ratio of root stimulating hormone was IBA 0. 75 mg/L,NAA 0. 05 mg/L and KT 0. 01 mg/L,and the rooting percentage was 66. 7%,but‘mihong＇varietie did not achieve the same effect. The papaya seedlings were respectively soaked in four different nutrient root water for 6 h by contrast experiment. It was determined the rooting water concentration was 50 mg/L to get the highest rate of seedling formation,and reaching 86. 1%. Fixed the best rooting water concentration,and the soaking time of 5 different gradients was tested. Without regard for time cost,finally,the highest percentage of seedlings was 8 hours for soaking,reaching 87. 5%. [Conclusion]The survival rates of two varieties of papaya were both almost the same.

关 键 词：番木瓜 组培苗 正交试验 生根率 成苗率 

分 类 号：S667.9[农业科学—果树学]