紫丁香苷抗肿瘤活性筛选及作用机制研究  

Study on the Screening and Mechanism of Syringin Anticancer Activity

作　　者：秦湫红 朱爱华[1,2,3] QIN Qiu- hong;ZHUAi- hua(Shaanxi New Drug Technology Development Center,Xi5 an,Shaanxi 710075;Shaanxi InnovativeDrug Research Center,Xi5 an,Shaanxi 710025;Key Laboratory of Research and Revelopment of Medicine of Shaanxi Province, Xi’ an,Shaanxi 710075)

机构地区：[1]陕西新药技术开发中心,陕西西安710075 [2]陕西省创新药物研究中心,陕西西安710075 [3]陕西省中药与天然药物研发重点实验室,陕西西安710075

出　　处：《安徽农业科学》2018年第14期107-108,112,共3页Journal of Anhui Agricultural Sciences

基　　金：陕西省青年科技新星计划项目(2016KJXX-31)

摘　　要：[目的]筛选对紫丁香苷较敏感的人肿瘤细胞系,研究其抗癌机理。[方法]采用四氮唑盐法(MTT法)测定药物的体外抑制作用,通过测定Caspase-3活性研究细胞凋亡通路在抗癌活性中的作用。[结果]在常见的15种人肿瘤细胞中,紫丁香苷的半数抑制浓度IC50低于100μg/m L的有人肝癌细胞HepG2以及人前列腺癌细胞PC-3,紫丁香苷可促进肿瘤细胞中细胞凋亡因子Caspase-3活性增加。[结论]人肝癌细胞HepG2以及人前列腺癌细胞PC-3对紫丁香苷较为敏感,紫丁香苷的抑制作用呈现出剂量依赖的特点;细胞凋亡途径在紫丁香苷的抗肿瘤活性中起着一定的作用。[Objective]To screen human tumor cell lines sensitive to syringin,and study its anticancer mechanism. [Method]To determine the inhibitory effect of syringin in vitro using tetrazolium salt（ MTT） assay,and study the role of apoptosis pathway by assay of Caspase-3 activity. [Result]Among the 15 common human tumor cell lines,syringin IC50 of hepatoma cell HepG2 and prostate cancer cell PC-3 were below 100 μg/m L. Syringin can promote the activity of Caspase-3 in tumor cells. [Conclusion] Hepatoma cell HepG2 and prostate cancer cell PC-3 are more sensitive to syringin,and the inhibition of syringin presents a dose-dependent manner. The cell apoptosis pathway plays a role in the anticancer activity of syringin.

关 键 词：紫丁香苷 抗肿瘤 细胞凋亡 

分 类 号：S567[农业科学—中草药栽培]