HPLC测定丛生竹中总黄酮及(异)牡荆苷、(异)荭草苷的含量  

Determination of Total Flavonoids and Vitexin,Isovitexin,Orientin,Isoorientin in Cluster Bamboo by High-performance Liquid Chromatography

作　　者：华梅[1,2] 陈剑[1,2] 毕玮[1,2] 孔继君[1,2] 呼延丽[1,2] 李云琴 杨宇明[1] 王娟[3] HUAMei;CHENJian;BIWei;KONG Ji-jun;HuYan-li;LI Yun-qin;YANG Yu-ming;WANG Juan(Yunnan Academy of Forestry,Kunming Yunnan 650201,P.R.China;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants/Key Laboratory for Conservation of Rare,Endangered ＆ Endemic Forest Plants,State Forestry Administration, Kunming Yunnan 650201,P.R.China;Southwest Forestry University,Kunming Yunnan 650224,P.R. China)

机构地区：[1]云南省林业科学院,云南昆明650201 [2]云南省森林植物培育与开发利用重点实验室/国家林业局云南珍稀濒特森林植物保护和繁育实验室,云南昆明650201 [3]西南林业大学,云南昆明650224

出　　处：《西部林业科学》2018年第3期86-90,95,共6页Journal of West China Forestry Science

基　　金：云南省科技计划项目(20152A005);云南省应用基础研究计划青年项目(2014FD069);云南省外专局资助"巴西实心竹繁殖与培育技术推广示范"项目;云南省中青年学术技术带头人后备人才培养项目(2010CI016);云南省森林植物培育与开发利用重点实验室开发基金

摘　　要：以勃氏甜龙竹竹叶为原料,对其中竹叶黄酮的超声提取工艺进行研究,同时采用高效液相色谱法(HPLC)对勃氏甜龙竹、箬竹、浦竹仔及椅子竹中总黄酮及牡荆苷、异牡荆苷、荭草苷、异荭草苷的含量进行测定。结果表明,勃氏甜龙竹超声提取最佳的工艺为:甲醇作为提取溶剂、浓度为70%、料液比为1︰10(w︰v)、超声时间60min、温度40℃。在最佳提取条件下,勃氏甜龙竹中总黄酮得率能达到4.63%。采用高效液相色谱-外标法测定4种丛生竹中总黄酮,含量分别为0.795 2%、1.166 9%、0.092 6%、0.897 2%。勃氏甜龙竹、箬竹和椅子竹中均含有牡荆苷、异牡荆苷、荭草苷及异荭草苷,浦竹仔中仅含有牡荆苷和异牡荆苷。分析方法的建立,为丛生竹化学资源的利用奠定基础。bThe extraction technique of bamboo flavonoids from Dendrocalamus brandisii（ Munro） Kurz leaves were studied by ultrasonic extraction technology. At the same time,the contents of total flavonoids and vitexin,isovitexin,orientin,isoorientin from Dendrocalamus brandisii（ Munro） Kurz,Indocalamus tessellatus（ Munro）Keng f.,Indosasa hispida Mc Clure and Dendrocalamus bambusoides Hsueh et D. Z. Li were determined by high performance liquid chromatography（ HPLC）. The results showed that the optimum ultrasonic process was methanol as extraction solvent,with 70% concentration,1 ︰ 10（ w ︰ v） material ratio,60 min ultrasonic time,40℃ temperature. Under the optimum conditions,the extraction rate of total flavonoids in Dendrocalamus brandisii（ Munro）Kurz was 4. 63%. The contents of total flavonoids in the four kinds of cluster bamboo were 0. 795 2%,1. 166 9%,0. 092 6% and 0. 897 2%,respectively. Dendrocalamus brandisii（ Munro） Kurz,Indocalamus tessellatus（ Munro）Keng f. and Dendrocalamus bambusoides Hsueh et D. Z. Li contain vitexin,isovitexin,orientin and isoorientin.Indosasa hispida Mc Clure contains only vitexin and isovite. The establishment of analytical methods laid the foundation for the utilization of chemical resources of cluster bamboo.

关 键 词：丛生竹 勃氏甜龙竹 HPLC 竹叶黄酮 超声提取 

分 类 号：S795[农业科学—林木遗传育种] O657.72[农业科学—林学]