甘氨酸对不同胁迫条件下高山离子芥试管苗的保护作用  

Protective effects of glycine on Chorispora bungeana plantlets under different stresses

作　　者：仇奕之 李宜珅 杨鹏军[1] 张旭强[1] 杨宁[1] QiuYi-zhi, Li Yi-shen, Yang Peng-jun, Zhang Xu-qiang, Yang Ning(College of Life Sciences, Northwest Normal University, Lanzhou 730070, Chin)

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《兰州大学学报（自然科学版）》2018年第2期200-207,共8页Journal of Lanzhou University(Natural Sciences)

基　　金：国家自然科学基金项目(31360061;31660116)

摘　　要：以高山离子芥试管苗为试验材料,测定冷胁迫、盐胁迫和干旱胁迫下,植株相对电导率、丙二醛浓度、脯氨酸质量分数、可溶性糖质量分数、过氧化物酶活性、超氧化物歧化酶活性、过氧化氢酶活性、磷脂酶D基因相对表达量和膜结合态磷脂酶D活性等生理指标,探究甘氨酸对不同胁迫条件下高山离子芥的保护作用.结果显示:甘氨酸对冷胁迫和干旱胁迫对植物造成的伤害均有一定的保护作用,对盐胁迫对植物造成的伤害则未见明显保护作用.Chorispora bungeana was used as experimental material, and its plantlets were treated with cold, salt and drought stresses. The relative electrolytic leakage, malondialdehyde content, proline content, soluble sugar content, catalase activity, superoxide dismutase activity, peroxidase activity, phospholipase D mRNA relative expression and the phospholipase D activity were measured to explore the protection of glycine on Chorispora bungeana under different stresses. The results showed that glycine could protect the Chorispora bungeana from cold and drought stress but salt stress.

关 键 词：甘氨酸 保护作用 胁迫 磷脂酶D 

分 类 号：Q945.78[生物学—植物学]