糖脂清方通过保护海马神经元对提高2型糖尿病大鼠学习记忆能力的影响  

Protective Effect of Tangzhiqing Formula on Learning and Memory Ability and Apoptosis of Hippocampal Neurons in Type 2 Diabetic Rats

作　　者：杨晓青 王旭 邵鑫 YANG Xiao-qing;WANG Xu;SHAO Xin(Clinical Hospital, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China)

机构地区：[1]南京中医药大学第一临床医学院

出　　处：《中国实验方剂学杂志》2018年第11期144-149,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省自然科学基金项目(BK20151572);南京中医药大学科技创新风险基金项目(CX201506)

摘　　要：目的：观察糖脂清方提高2型糖尿病大鼠学习记忆能力,保护海马神经元的作用。方法：SD雄性大鼠,采用高糖高脂饲料加链脲佐菌素（30 mg·kg^-1）腹腔注射制作2型糖尿病模型。将造模大鼠随机分为模型组、糖脂清高、中、低（12,6,3 g·kg^-1）剂量组,另设正常组。连续灌胃8周后进行水迷宫行为学测试,结束后处死取材。苏木-伊红（HE）染色观察海马组织病理形态学结构,原位末端转移酶标记技术（TUNEL）检测海马神经元细胞凋亡情况。结果：与模型组比较,糖脂清方能明显降低2型糖尿病大鼠血糖（P〈0.05,P〈0.01）,明显缩短逃避潜伏期,增加穿越平台次数、原平台游泳路程及游泳时间（P〈0.05,P〈0.01）;显著改善海马组织病理情况,更加显著地降低海马神经元细胞凋亡指数（P〈0.01）。结论：糖脂清方可以显著降低高糖高脂饮食加小剂量链脲佐菌素诱导的2型糖尿病大鼠模型的血糖水平,改善胰岛素抵抗,提高大鼠学习记忆能力,减少海马神经元细胞凋亡,并具有保护作用。Objective： This experiment was used to investigate the protective effect of Tangzhiqing formula(TZQ）,clearing prescription on learning,memory ability and apoptosis of hippocampal neurons in type 2 diabetic rats. Method： SD male rats were fed with high glucose and high fat diet and streptozotocin(STZ）(30 mg·kg^-1） intraperitoneal injection to produce type 2 diabetes mellitus model. The model rats were randomly divided into model group,high,middle and low dose group,the daily dose was 12,6,3 g·kg^-1,compared with normal control group. After 8 weeks, the Morris maze test was conducted. The pathological structure of hippocampus was observed by hematoxylin-eosin(HE） staining,and the apoptosis of hippocampal neurons was detected by Td T-mediated d UTP nick end labeling(TUNEL） staining. Result： Compared with the model group,TZQ could significantly reduce the blood glucose of type 2 diabetic rats(P〈0. 05,P〈0. 01）; reduce latency of escape(P〈0. 05,P〈0. 01）; increase the number of crossing platform,the platform of swimming distance and swimming time(P〈0. 05, P〈0. 01）; improve pathological of hippocampus tissue; significantly reduce the apoptotic index of hippocampal neurons(P〈0. 01）. Conclusion： TZQ could significantly reduce the high glucose of type 2 diabetes model rats fed with high fat diet and injected low dose streptozotocin. TZQ improved insulin resistance,learning and memory ability in rats. TZQ also could protect hippocampal neurons and reduce apoptosis.

关 键 词：糖脂清方 糖尿病 认知功能障碍 海马神经元 凋亡 

分 类 号：R22[医药卫生—中医基础理论] R24[医药卫生—中医学]