含HOE642的新型器官保存液对离体兔肺移植供肺细胞凋亡的影响  

Effect of a new organ preservation solution with HOE642 on the apoptosis of the donor lung from a modified lung transplantation model of rabbits

作　　者：余得水[1,2] 龚添庆[2] 周文琴[2] 郑剑桥 刘斌[2] YU Deshui 1,2, GONG Tianqing2, ZHOU Wenqin2, ZHENG Jianqiao 2, LIU Bin2(1. Department of Anesthesiology, the Second People＇s Hospital of Yibin ,Yibin, Sichuan 644000, China;2. Department of Anesthesiology,West China Hospital of Sichuan University, Chengdu, Sichuan 610068, Chin)

机构地区：[1]四川省宜宾市第二人民医院麻醉科,644000 [2]四川大学华西医院麻醉科,成都610068

出　　处：《重庆医学》2018年第13期1709-1711,1715,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(30972862)

摘　　要：目的探索含Ⅰ型钠氢通道拮抗剂HOE642的新型器官保存液对离体兔肺移植供肺细胞凋亡的影响。方法 24只雄性成年新西兰大白兔分2组[低钾葡聚糖(LPD)组和HOE组],分别用LPD液及新型器官保存液灌洗供肺,建立兔改良离体单肺移植模型并再灌注2h。测定供肺细胞凋亡指数、caspase-3、Fas/FasL及Bax/Bcl-2表达水平。结果与LPD组比较,HOE组供肺细胞凋亡指数和caspase-3的阳性细胞计数明显下降(P<0.05),Fas、Fas-L、Bax表达水平明显降低,Bcl-2表达升高(P<0.05)。结论含HOE642的新型器官保存液可通过抑制细胞内外源性凋亡途径抑制细胞凋亡。Objective To explore the effect of a new organ preservation solution with HOE642 on the apoptosis of the donor lung from a modified lung transplantation model of rabbits. Methods 24 male rabbits were divided into two groups [low potassium dextran （LPD） group and HOE group] ,established rabbit models for next 2-h reperfusion using LPD solution or new organ preservation solution. Detected the levies of apoptosis index and caspase-3,the expression of Fas/Fas-L and Bel-2/Bax. Results Compared with LPD group, HOE group revealed significant lower level of apoptosis index and caspase-3 （P〈0.05）,lower expression of Fas/Fas-L and Bax,and higher expression of Bcl-2 （P〈0.05）. Conelusion The potential donor lung protective mechanism offered by the new organ preservation solution with HOE642 might be the inhibition of apoptosis via both intrinsic and extrinsic pathways.

关 键 词：器官保存液 肺移植 细胞凋亡 HOE642 

分 类 号：R655.3[医药卫生—外科学]