miRNA-129-5p干扰技术对胆道闭锁小鼠胆道EMT作用机制的研究  

Study on the mechanism of miRNA-129-5p in epithelial-mesenchymal transition of biliary atresia mouse

作　　者：王建尧[1] 王斌[1] 吴宙光[1] 叶晓烁[1] 陈子民[1] 冯奇[1] 刘冬[1] 姚君 WANG Jianyao1 ,WANG Bin1 ,WU Zhouguang1 ,YE Xiaoshuo1 , CHEN Zimin 1 , FENG Qi1 , LIU Dong1, YAO Jun2(1. Department of General 518000,China; 2. Surgery Ward Ⅱ , Shenzhen Children＇s Hospital ,Shenzhen,Guangdong Department of Gastroenterology, Shenzhen People＇s Hospital, Shenzhen , Guangdong 518000, Chin)

机构地区：[1]广东省深圳市儿童医院普外二科,518000 [2]广东省深圳市人民医院消化内科,518000

出　　处：《重庆医学》2018年第13期1723-1726,共4页Chongqing medicine

基　　金：广东省医学科研基金(B2014360);广东省深圳市科技创新委员会科技计划项目(JCYJ20160429174927286;JCYJ20150403101028164)

摘　　要：目的探讨miRNA-129-5p在胆道闭锁上皮间质转化(EMT)中的作用机制。方法构建胆道闭锁小鼠模型(实验组),取实验组胆道组织,构建TGF-β1诱导的胆管上皮EMT细胞模型,检测TGF-β1诱导刺激后胆道上皮细胞株EMT相关标志物表达水平,并检测miRNA-129-5p的表达变化。同时转染miRNA-129-5p的前体,比较转染前后TGF-β1刺激下胆道上皮细胞EMT相关标志物及细胞外基质的表达改变。结果实验组小鼠肝脏肝外胆道呈EMT样改变,miRNA-129-5p在实验组小鼠中的表达水平下调(P<0.05),过表达miRNA-129-5p可以抑制EMT的进程(P<0.05)。结论 miRNA-129-5p可能通过TGF-β1通路调控胆道上皮EMT的进程。Objective To investigate the mechanism of miRNA-129-5p in epithelial-mesenchymal transition （EMT）of biliary atresia. Methods Constructed bile duct epithelial EMT cell model （the experimental group） induced by TGF-β1,detected the expressions of EMT related markers and miRNA-129-5p. While miR- NA-129-5p precursor was transfected,the expressions of EMT related markers and extracellular matrix were contrasted between the original and the renovated biliary epithelial cells. Results In the experimental group, extrahepatic bile duct showed EMT,the expression of miRNA-129-Sp was decreased （P〈0.05）, overexpression of miRNA-129-Sp could inhibit the progression of EMT （P〈0.05）. Conclusion miRNA-129-5p may relate to EMT by regulating, the expression of TGF-β1.

关 键 词：胆道闭锁 miRNA-129-5p 上皮间质转化 

分 类 号：R726.5[医药卫生—儿科]