Isolation and Identification of Pathogenic Bacteria Causing Bovine Subclinical Mastitis in Eastern Hebei Province  

作　　者：Gao Guisheng Wu Tonglei Zhang Zhiqiang Yang Zongze Fu Zhixin Zou Fengping Gao Guangping An Jiwei Yao Weiping Shi Qiumei 

机构地区：[1]Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology ,Changli 066600,China [2]Qian'an Bureau of Agriculture, Animal Husbandry and Fisheries,Qian'an 064400,China [3]Animal Husbandry and Veterinary Bureau of Leting County,Leting 063602,China [4]Animal Husbandry and Fisheries Bureau of Funing District,Qinhuangdao 066300,China

出　　处：《Animal Husbandry and Feed Science》2018年第1期65-67,共3页动物与饲料科学（英文版）

基　　金：Supported by Beef Cattle Disease Prevention and Control Project of Agricultural Industry Technology System of Hebei Province;The Fund of Qinhuangdao Science and Technology Bureau(200901A070);China Spark Program(2012GA620002)

摘　　要：In this study, the milk samples of 1 021 cows in eight dairy farms in Eastern Hebei Province were collected and detected with LMT reagent and somatic cell count for subclinical mastitis. Pathogenic bacteria in subclinical mastitis positive milk samples were isolated and identified.The results showed that 60.63%（619/1 021） of the sampled cows were diagnosed with subclinical mastitis, and mixed infections accounted for 88.21%（546/619） of the cases. In addition, 82 strains of 14 species were isolated from the subclinical mastitis positive milk samples, including 36 strains of Staphylococcus（43.90%）, 33 strains of Streptococcus（40.24%）, 8 strains of Enterobacteriaceae（9.76%） and 5 strains of Corynebacterium（6.10%）, respectively. The results proved that Staphylococcus aureus and Streptococcus agalactiae are the main pathogenic bacteria causing bovine subclinical mastitis in Eastern Hebei Province.In this study, the milk samples of 1 021 cows in eight dairy farms in Eastern Hebei Province were collected and detected with LMT reagent and somatic cell count for subclinical mastitis. Pathogenic bacteria in subclinical mastitis positive milk samples were isolated and identified.The results showed that 60.63%（619/1 021） of the sampled cows were diagnosed with subclinical mastitis, and mixed infections accounted for 88.21%（546/619） of the cases. In addition, 82 strains of 14 species were isolated from the subclinical mastitis positive milk samples, including 36 strains of Staphylococcus（43.90%）, 33 strains of Streptococcus（40.24%）, 8 strains of Enterobacteriaceae（9.76%） and 5 strains of Corynebacterium（6.10%）, respectively. The results proved that Staphylococcus aureus and Streptococcus agalactiae are the main pathogenic bacteria causing bovine subclinical mastitis in Eastern Hebei Province.

关 键 词：Dairy cow Subclinical mastitis Pathogen Isolation and identification 

分 类 号：S858.23[农业科学—临床兽医学] TS252.1[农业科学—兽医学]