无牙颌患者口腔菌群组成的焦磷酸测序分析  

Pryosequencing analysis of the compositions of oral flora from edentulous individuals

作　　者：张松梅[1] 田菲 张富强[1] 郭晓奎 邱憬[3] ZHANG Songmei, TIAN Fei, ZHANG Fuqiang, GUO Xiaokui, QIU Jing.(Department of Prosthodontics, Shanghai Key Laboratory of Stomatology , Shanghai Ninth People＇s Hospital, Shanghai 200011, Chin)

机构地区：[1]上海交通大学医学院附属第九人民医院口腔修复科上海市口腔医学重点实验室,上海200011 [2]上海交通大学医学院病原生物学教研室,上海200025 [3]南京医科大学附属口腔医院种植科南京医科大学口腔疾病研究江苏省重点实验室,江苏南京210029

出　　处：《口腔医学》2018年第5期389-393,共5页Stomatology

基　　金：国家自然科学基金(81472928);江苏省卫生计生委面上科研课题(H201641);江苏省"六大人才高峰"资助项目(2014-WSW-035);江苏省青年医学人才项目(QNRC2016850);东南大学-南京医科大学合作基金项目(2242017K3DN14);江苏高校优势学科建设工程资助项目(2014-37)

摘　　要：目的研究无牙颌患者的口腔菌群组成。方法收集11例无牙颌患者口底/舌腹、舌背、唇颊前庭、硬腭/牙槽嵴顶、唾液中的菌斑样本,构建细菌16S rRNA基因文库,采用焦磷酸测序法对16S rRNA基因的V2-V3可变区进行序列分析,完成无牙颌患者口腔菌群的物种鉴定。结果 11例无牙颌患者共测得51 091条16S rRNA基因序列,主要分布于厚壁菌门、变形菌门、拟杆菌门、放线菌门,多为链球菌属、奈瑟菌属、卟啉单胞菌属、罗氏菌属等,优势菌种为缓症链球菌、毗邻颗粒链菌、殊异韦荣菌、香茅醇假单胞菌、副猪嗜血杆菌、动物口腔奈瑟氏球菌、卟啉单胞菌、黏液罗氏菌。结论牙列缺失对无牙颌患者的口腔菌群组成有一定影响。Objective To explore the compositions of oral flora from edentulous individuals. Methods Bacteria plaque samples were collected from mouth floor/tongue abdomen,tongue back,labial/buccal vestibule,hard palate/alveolar ridge crest and saliva of 11 edentulous individuals. The microbial 16S rRNA gene clone libraries were then constructed. Using pryosequencing,sequencing analysis of the V2-V3 variable zone of 16S rRNA was performed to execute species identification of oral flora from 11 edentulous individuals. Results A total of 51 091 sequences,which were classified into Firmicutes,Proteobacteria,Bacteroidetes and Actinobacteria phyla,were identified by pyrosequening in the 11 edentulous subjects. Predominant genera included Streptococcus,Neisseria,Porphyromonas,Rothia,etc. Streptococcus mitis,Granulicatella adiacens,Veillonella dispar,Pseudomonas citronellolis,Haemophilus pittmaniae,Neisseria zoodegmatis,Porphyromonas sp and Rothia mucilaginosa were dominant species. Conclusion Complete absence of dentitions has an impact on the oral flora compositions of edentulous individuals.

关 键 词：无牙颌 口腔菌群 焦磷酸测序法 

分 类 号：R37[医药卫生—病原生物学]