马归液对腺性膀胱炎大鼠模型Survivin、PTEN表达的影响  

Effect of Magui Liquid on the Expression of Survivin and PTEN in Rats with Cystitis Glandularis

作　　者：蔡蔚[1] 谢海平[1] 龙蠡[1] 谢晓[1] 杨华伟[1] CAI Wei, XIE Hai-ping, LONG Li, XIE Xiao, and YANG Hua-wei(Department of Urology, First Affiliated Hospital of TCM University of Hunan, Changsha（410007）)

机构地区：[1]湖南中医药大学第一附属医院泌尿外科,长沙410007

出　　处：《中国中西医结合杂志》2018年第5期558-563,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：湖南省教育厅科研优秀青年项目(No.15B175);湖南省中医药管理局重点项目(No.201728)

摘　　要：目的观察马归液对腺性膀胱炎大鼠模型膀胱组织Survivin、PTEN表达水平的影响。方法将40只腺性膀胱炎模型大鼠随机分成4组:生理盐水组、吡柔比星组、马归液1组、马归液2组,每组10只。分别灌注0.2mL生理盐水、吡柔比星、马归液(每周1次)、马归液(每周2次),连续膀胱灌注8周。取各组大鼠膀胱组织进行病理学检查、透射电镜观察,并采用免疫组化和Real-time PCR方法检测Survivin、PTEN表达情况。结果病理和透射电镜的结果显示,马归液可以抑制炎症细胞和修复细胞的损伤。与生理盐水组比较,吡柔比星组和马归液1、2组的PTEN平均灰度值及mRNA表达升高(P<0.01),Survivin平均灰度值及mRNA表达降低(P<0.01)。与吡柔比星组比较,马归液1组PTEN平均灰度值及mRNA表达降低(P<0.05),Survivin平均灰度值及mRNA表达升高(P<0.05);与马归液1组比较,马归液2组的PTEN平均灰度值及mRNA表达升高(P<0.05),Survivin平均灰度值及mRNA表达降低(P<0.05)。结论马归液可提高PTEN并抑制Survivin表达,作用与吡柔比星相近,膀胱灌注每周2次效果更佳。Objective To observe the effect of Magui Liquid on the expression of Survivin and PTEN in bladder tissue of cystitis glandularis model rats. Methods Totally 40 cystitis glandularis model rats were randomly divided into saline group,pirarubicin group,Magui Liquid 1 group and Magui Liquid 2 group,10 rats in each group. T he rats were treated with 0.2 mL normal saline,pirarubicin solution,Magui Liquid once a week） and Magui Liquid（twice a week） for 8 weeks respectively by intravesical instillation. The bladder tissues of rats in each group were examined by pathological examination and transmission electron microscopy,and the expression of Survivin and PTEN were detected by immunohistochemistry and Real-time PCR. Results The results of pathology and transmission electron microscopy showed that Magui Liquid could inhibit the injury of inflammatory cells and repair cells. Compared with saline group, the average gray values and mRNA expression of PTEN in Magui Liquid 1,2 group and pirarubicin group increased（P〈0.01）,the average gray values and mRNA expression of Survivin in Magui Liquid 1, 2 group and pirarubicin group decreased（P〈0.01）. Compared with pirarubicin group,the average gray values and mRNA expression of PTEN in Magui Liquid 1 group decreased（P〈0.05）, and the average gray values and mRNA expression of Survivin increased（P〈0.05）. Compared with Magui Liquid 1 group,the average gray values and mRNA expression of PTEN in Magui Liquid 2 group increased（P〈0.05）, and the average gray values and mRN A expression of Survivin decreased（P〈0.05）. Conclusions Magui Liquid can increase mRN A expression of PTEN and decrease mRNA expression of Survivin. Its effect is similar to that of pirarubicin.The effect of the intravesical instillation of Magui Liquid twice a week is better.

关 键 词：腺性膀胱炎 马归液 SURVIVIN PTEN 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]