半乳糖凝集素3在糖尿病足溃疡患者中的表达  

Expression of galectin-3 in patients with diabetic foot ulcer

作　　者：邹译娴[1] 李国庆 汤参娥[3] 孟莉娟 ZOU Yi-xian , LI Guo-qing, TANG Can-e, MENG Li-juan(The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, Chin)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]南华大学药学与生物科学学院、湖南省分子靶标新药研究协同创新中心 [3]中南大学湘雅医院医学科学研究中心 [4]南华大学附属第二医院

出　　处：《实用预防医学》2018年第6期649-651,共3页Practical Preventive Medicine

基　　金：国家自然科学基金(81570776)

摘　　要：目的探讨糖尿病足溃疡(diabetic foot ulcer,DFU)患者伤口和血清中半乳糖凝集素3(galectin-3)的表达,以期阐明galectin-3和DFU的关系。方法选取2015年1月-2016年3月Wanger 1、2级的糖尿病足患者50例,另选取同期血糖正常的外伤患者20例为对照,分别取其足部伤口边缘组织及血清。对所有入选对象均行ELISA检测血清galectin-3浓度,Western-blot法检测伤口边缘组织galectin-3的表达水平。结果 ELISA结果显示DFU组血清galectin-3水平高于外伤对照组[(5.66±2.76)vs(4.25±1.94)ng/ml](P=0.04),Western-blot结果显示galectin-3在DFU组伤口边缘组织表达增强。结论 galectin-3在DFU患者中高表达,galectin-3的高表达可能参与了糖尿病足的进展。Objective To investigate the expression of galectin-3 in wounds and sera of patients with diabetic foot ulcer（ DFU）so as to clarify the correlation between galectin-3 and DFU. Methods Fifty DFU patients with Wagner 1-2 stages and 20 wounded patients with normal blood glucose level（ serving as the control group） were enrolled to obtain their tissues from the edge of the foot wound and sera from January 2015 to March 2016. The serum concentration of galectin-3 was detected by ELISA,and the expression of galection-3 in the tissues was detected by Western-blot. Results ELISA results showed that the serum concentration of galectin-3 was higher in the DFU group than in the wounded control group（（ 5.66± 2.76） ng/m L vs.（ 4.25± 1.94）ng/m L,P = 0.04）. Western-blot results indicated that the expression of galectin-3 in tissues from wound edge of the DFU group was enhanced. Conclusions Galectin-3 is highly expressed in the patients with DFU,and its high expression may contribute to the development of DFU.

关 键 词：GALECTIN-3 血清 糖尿病足 

分 类 号：R587.1[医药卫生—内分泌]