流式细胞仪检测纳帕海高原湿地浮游病毒和浮游细菌丰度  

Determination of virioplankton and panktonic bacteria abundance in the Napahai plateau wetland by flow cytometry

作　　者：张中耀 王爽 孙策[1] 魏云林[1] 林连兵[1] 张琦[1] 季秀玲[1] ZHANG Zhong-Yao, WANG Shuang, ZHANG Qi, SUN Ce, WEI Yun-Lin, LIN Lian-Bing, JI Xiu-Ling(Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, Chin)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《微生物学通报》2018年第5期1007-1015,共9页Microbiology China

基　　金：国家自然科学基金(31700324);云南省教育厅科学研究基金(2017ZZX132);昆明理工大学校人培基金(KKZ3201526010)~~

摘　　要：【背景】浮游病毒是水体微生物群落中重要的组成成分,深入研究浮游病毒的时空分布有助于更好地保护和开发当地的微生物资源。【目的】对采集到的纳帕海高原湿地水样中的浮游病毒和浮游细菌进行计数,揭示纳帕海高原湿地浮游病毒的分布规律。【方法】采用流式细胞仪检测2013年12月和2014年9月纳帕海高原湿地7个水样的浮游病毒与浮游细菌丰度,并对影响浮游病毒丰度的因素,如细菌丰度、叶绿素a含量以及其他环境因子进行了相关性分析。【结果】季节分布上,雨季浮游病毒和浮游细菌丰度高于旱季;水平分布上,原水样品的浮游病毒高于湿地水和淤泥水。旱季水样的浮游病毒丰度受到细菌丰度及叶绿素a浓度的影响较大;雨季水样的浮游病毒丰度受到水体的p H值和温度的影响较大。【结论】纳帕海高原湿地的浮游病毒和浮游细菌是比较活跃的。浮游病毒丰度在不同季节、不同采样点受到细菌丰度和叶绿素a浓度等因素的不同影响。在旱季噬菌体而非噬藻体或浮游植物病毒是纳帕海高原湿地中浮游病毒的优势种群。[Background] Virioplankton is one of the most important components of microbial communities. The study on the temporal and spatial distribution of the virioplankton contributes to the protection and development of local microbial resources. [Objective] Virioplankton and planktonic bacteria of water samples were counted, aiming to investigate the virioplankton distribution in the Napahai plateau wetland. [Methods] Water samples from seven sites were obtained in December 2013 and September 2014. Virioplankton and planktonic bacteria abundance were determined using flow cytometry. Here we reported on virioplankton relationships with environmental factors, such as bacterial abundance, chlorophyll a concentration and other environmental factors. [Results] Virioplankton and planktonic bacteria abundance in rainy season was higher than that of in dry season at the seasonal distribution. Virioplankton abundance in dry season was influenced by bacteria abundance and chlorophyll a concentration; whereas virioplankton abundance in the rainy season was influenced by p H and temperature. [Conclusion] We found high virioplankton and planktonic bacteria abundance values. Virioplankton abundance was correlated with bacterioplankton abundance and chlorophyll a concentration in different seasons and sampling points. The phage, rather than the algal or phytoplankton viruses, was the dominant population in dry season of the Napahai plateau wetland.

关 键 词：浮游病毒 浮游细菌 流式细胞仪 叶绿素A 

分 类 号：Q938.8[生物学—微生物学]