2型糖尿病患者ABCA1、PPARγ、SREBP、ADPN、LXRα表达水平及临床价值研究  

Study on the expression of ABCA1,PPAR,SREBP,ADPN and LXR α in patients with type 2 diabetes mellitus

作　　者：李观强[1] 杨辉[1] 洪雄新[1] 万彦彬[1] 李小峰[1] 郭中秋[1] 张秋霞[1] 杨素珍[1] LI Guanqiang;YANG Hui;HONG Xiongxin;WAN Yanbin;LI Xiaofeng;GUO Zhongqiu;ZHANG Qiuxia;YANG Suzhen(Department of Clinical Laboratory,People＇s Hospital of Longgang District of Shenzhen , Shenzhen ,Guangdong 518000, China)

机构地区：[1]深圳市龙岗区人民医院检验科,广东深圳518000

出　　处：《国际检验医学杂志》2018年第10期1199-1201,1205,共4页International Journal of Laboratory Medicine

基　　金：广东省深圳市龙岗区科技计划项目(YLWS20150513171755606)

摘　　要：目的探讨2型糖尿病患者中三磷酸腺苷(ATP)结合盒转运体A1(ABCA1)、过氧化物酶体增殖物激活受体(PPARγ)、固醇调节元件结合蛋白(SREBP)、脂联素(ADPN)、小鼠肝X受体α(LXRα)表达水平及其临床价值。方法选自该院于2015年6月至2017年6月期间收治的2型糖尿病患者71例作为观察组;另选自该院于2015年6月至2017年6月期间健康体检者60例作为对照组。清晨空腹采集受试者外周静脉血,分离血清,采用放射免疫法测定血清人ADPN含量,采用酶联免疫吸附双抗体夹心法测定血清ABCA1、PPARγ、SREBP及LXRα含量。结果观察组血清ABCA1和ADPN含量低于对照组,而血清PPARγ、SREBP和LXRα含量高于对照组,差异均有统计学意义(P<0.05);ABCA1+PPARγ+SREBP+ADPN+LXRα五项联合检测灵敏度和特异度高于ABCA1、PPARγ、SREBP、ADPN和LXRα单独检测。结论 2型糖尿病患者ABCA1和ADPN表达下降,而PPARγ、SREBP和LXRα表达上升,ABCA1+PPARγ+SREBP+ADPN+LXRα五项联合检测具有较高的灵敏度和特异度,具有重要的临床研究价值。Objective To investigate the value of the expression of Adenosine Triphosphate（ATP）binding cassette transporter A1（ABCA1）,peroxisome proliferator activated receptorγ（PPARγ）and sterol regulatory element binding protein（SREBP）,adiponectin（ADPN）and liver X receptoα（LXRα）in type 2 diabetic patients.Methods 71 patients with type 2 diabetes received in the hospital from June 2015 to June 2017 were selected as the observation group,and 60 healthy persons who underwent the health assessment from June 2015 to June 2017 were selected as the control group.Peripheral venous blood was collected from patients with an empty stomach in the morning,serum was isolated and serum human ADPN content were measured by radioimmunoassay.The levels of serum ABCA1,PPARγ,SREBP and LXRαwere measured by Enzyme linked immunosorbent assay.Results The serum levels of ABCA1 and ADPN in the observation group were lower than those in the control group,while serum PPARγSREBP and LXRαlevels were higher than those in the control group（P0.05）;the diagnostic sensitivity and specificity of ABCA1＋PPARγ＋SREBP＋ADPN＋LXRαwere higher than those of single detection of ABCA1,PPARγ,SREBP,ADPN and LXRα.Conclusion The levels of ABCA1 and ADPN decreased in patients with type 2 diabetes,while the levels of PPARγ,SREBP and LXRαwas increased.The five joint diagnosis of ABCA1＋PPARγ＋SREBP＋ADPN＋LXRαhas high sensitivity and specificity.It was of important clinical value and worth further application.

关 键 词：2型糖尿病 ATP结合盒转运体A1 过氧化物酶体增殖物激活受体 固醇调节元件结合蛋白 脂联素 小鼠肝X受体α 

分 类 号：R587.1[医药卫生—内分泌]