TNF-α对大鼠皮层神经元钠通道电流的影响  

Effects of TNF-α on sodium channel in cortical neurons of rats

作　　者：张伟伟[1] 张晗[1] 曹芳[1] 穆淑梅[1] 康现江[1] ZHANG Weiwei;ZHANG Han;CAO Fang;MU Shumei;KANG Xianjiang(College of Life Sciences,Hebei University,Baoding 071002,China)

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2018年第3期278-283,298,共7页Journal of Hebei University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31572269);生物学河北省国家重点学科培育项目;河北省教育厅资助项目(QN2017021);河北省卫生与计划生育委员会资助项目(20180702);河北省高等学校科学技术研究项目(Z2014063)

摘　　要：以原代培养的胎鼠皮层神经元为材料,使用全细胞膜片钳技术,研究了10ng/mL与50ng/mL的肿瘤坏死因子(tumor necrosis factor-α,TNF-α)分别处理6h和24h后对皮层神经元电压依赖性钠电流的影响.结果显示,50ng/mL的TNF-α对钠电流具有显著抑制作用,而对钠通道的激活性质与失活性质没有影响.Primary cultured fetal rat cortical neurons and whole cell patch clamp technique were used for investigating the effect of TNF-αon Na＋currents of cultured cortical neurons.The neurons were treated for 6 and 24 hours respectively by TNF-α（10 and 50 ng/mL）.The results showed the inhibition of 50 ng/mL TNF-α on voltage-gated Na~＋ currents in cortical neurons of rats,but has no effect on the activation properties and the inactivation properties of sodium channel.

关 键 词：肿瘤坏死因子α(TNF-α) 皮层神经元 电压依赖性钠通道 膜片钳记录 

分 类 号：Q25[生物学—细胞生物学] Q42