高压氧治疗对高原脱习服症大鼠主要脏器超微结构的影响  

Effects of hyperbaric oxygen treatment on ultrastructure of rats with plateau deacclimatization

作　　者：崔宇 吴佩锋 徐钢 崔建华[2] CUI Yu;WU Peifeng;XU Gang;CUI Jianhua(Faculty of High Altitude Military Medicine,Army Military Medical University,Chongqing 400038;Research Center for Prevention and Treatment of High Altitude Diseases of Xinjiang Military Region,Yecheng,Xinjiang 844900,China)

机构地区：[1]陆军军医大学高原军事医学系,重庆400038 [2]新疆军区高山病防治研究中心,新疆叶城844900

出　　处：《重庆医学》2018年第14期1841-1844,1849,共5页Chongqing medicine

基　　金：国家科技支撑计划项目(2009BAI85B05);高原医学教育部重点实验室资助项目(2011JSGY05);全军"十一五"医药卫生科研基(06MA091)

摘　　要：目的探讨高压氧(HBO)治疗对高原脱习服症大鼠心、脑、肺组织超微结构的影响。方法选择SD大鼠49只,44只在模拟海拔5 000m的低压氧舱常规喂养3个月,在低压舱内死亡9只,将35只成活大鼠出舱后按体质量随机分为高原对照组(出舱后即刻)、HBOⅠ组(HBO治疗后7d)、HBOⅡ组(HBO治疗后1个月)、HBOⅢ组(HBO治疗后2个月)、对照组Ⅰ组、对照Ⅱ组、对照Ⅲ组,每组5只。HBOⅠ、Ⅱ、Ⅲ组给予HBO治疗7d;对照组Ⅰ、Ⅱ、Ⅲ组出低压氧舱后同步在常压常氧下喂养,与HBOⅠ、Ⅱ、Ⅲ组同步处死;余下5只在常氧常压下喂养作为平原对照组。各组大鼠在相应时间内快速处死取出额叶脑皮质、心、肺组织标本,在光镜和电镜下观察各组超微结构的变化。结果低氧引起脑神经细胞线粒体肿胀、脊断裂、线粒体和周围结构水肿;心肌细胞浊肿、空泡变性,肌丝排列不规则,炎性细胞浸润,血管扩张;肺泡隔增宽,毛细血管扩张充血,肺泡腔内有粉红色渗出物,炎性细胞浸润,肺泡壁增厚。HBO治疗后,组织超微结构损伤较对照组明显改善,HBOⅡ组恢复明显好于HBOⅠ组,HBOⅢ组明显好于HBOⅡ组,2个月完全恢复到平原水平。结论 HBO治疗具有改善缺氧大鼠脑神经损伤、促进细胞的功能恢复,降低缺氧引起的肺动脉高压,对缺血心肌具有保护作用。Objective To research the effects of hyperbaric oxygen（HBO）on the ultrastructure of heart,brain and lung in rats with plateau deacclimatization.Methods Forty-mine SD rats were selected.Fortyfour SD rats were routinely fed in a hypobaric hypoxia chamber at the simulated altitude of 5 000 m for 3 months.Nine rats died in the hypobaric hypoxia chamber.After coming out from the chamber,35 survival rats were randomly divided into the high altitude control group（instantly after out of the hypobaric hypoxia chamber）,HBO groupⅠ,Ⅱ and Ⅲ（7 days,1 months and 2 months after HBO treatment）and control groupsⅠ,Ⅱ and Ⅲ according to the body mass,5 cases in each group;the HBO groups were treated by HBO for 7 d.The control group Ⅰ,Ⅱ and Ⅲ were synchronously fed in normal pressure and oxygen environment after HBO treatment.The rats in all groups were synchnonously sacrificed,the other 5 SD rats as the plain control group were fed in hormal pressure oxygen environment.The frontal cortex,heart and lung tissues were taken out for observing the ultrastructure changes by optical microscopy and electron microscopy.Results Hypoxia induced mitochondria swelling and ridge breakage in cranial nerves cells,swelling of mitochondria and peripheral structures,myocardial cells cloudy swelling,vacuolar degeneration,muscle fibers irregular arrangement,inflammatory cell infiltration and vasodilatation.The alveolar septm was broadened,blood capillaries were dilated and congestive,alveolar space was filled with pink transudation,inflammatory cells were infiltrated,and alveolar wall was incrassated.After HBO treatment,the tissue ultrastructure injurys were significantly improved compared with the control group,the recovery in the HBO group Ⅱ was significantly better than that in the HBO groupⅠ,and the HBO group Ⅲ was significantly better than the HBO groupⅡ,which recovered to plain level after 2 months.Conclusion HBO treatment has the effects for improving cranial nerves injury in hypoxia rat,promotes cellular func

关 键 词：高原脱习服症 高压氧 组织超微结构 

分 类 号：R339.5[医药卫生—人体生理学]