两种方法建立SD大鼠黄褐斑模型的比较实验研究  

Comparative experiment study of two kinds of methods for establishing SD rat chloasma model

作　　者：邓志博 何施逸 王乾力 李韫韬 胡乔莉 杜永洪[1] DENG Zhibo;HE Shiyi;WANG Qianli;LI Yuntao;HU Qiaoli;DU Yonghong △(State Key Laboratory Culture Base of Biomedical Engineering Co-built by Chongqing Medical University and Ministry of Science and Technology/Chongqing Key Laboratory of Ultrasound Medicine Engineering/Chongqing Municipal Key Laboratory of Biomedical Engineering,Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学生物医学工程学院省部共建国家重点实验室培育基地-重庆市超声医学工程重点实验室/重庆市生物医学工程学重点实验室,重庆400016

出　　处：《重庆医学》2018年第15期1993-1996,共4页Chongqing medicine

基　　金：重庆市基础科学与前沿技术研究专项(csct2016jcyjA0098)

摘　　要：目的对比分析黄体酮注射液和紫外线照射两种方法建立SD大鼠黄褐斑模型。方法分别采用高低剂量的黄体酮药物注射和紫外线辐照建立黄褐斑大鼠模型。观察斑块面积及光镜下观察皮肤切片,检测与黄褐斑黑色素沉积密切相关的超氧化物歧化酶(SOD)及丙二醛(MDA)水平。结果肉眼观察高剂量黄体酮组大鼠的背部皮肤可见显著色素沉积,低剂量黄体酮组可见散在色素沉积;紫外线组均可见明显色素沉积。与对照组比较,高剂量黄体酮组大鼠MDA水平升高、SOD水平降低,低剂量黄体酮组大鼠血清与皮肤组织中的SOD水平降低,差异有统计学意义(P<0.05)。高剂量紫外线组大鼠血清和皮肤组织中的SOD水平降低、MDA水平升高,低剂量组紫外线的大鼠皮肤组织中的SOD水平降低(P<0.05),但两组比较差异无统计学意义(P>0.05)。光镜下可见建模组黑色素颗粒成团堆积成黑色小体,黄体酮组堆积杂乱分布,其中高、低剂量组间有明显区别;紫外线组成线性密集排列,组间无显著差异。结论黄体酮注射液和紫外线照射均能成功建立SD大鼠黄褐斑模型,紫外线照射建模效果和稳定性较好。Objective To comparatively analyze the SD rat chloasma model established by using the two methods of progesterone injection and ultraviolet radiation. Methods The rat chloasma model was established by adopting the high and low doses of progesterone injection and ultraviolet irradiation.The plaque area was observed and the skin section was observed by light microscope.The SOD and MDA levels closely related with chloasma melanin deposition were detected. Results The significant pigment deposition on the back skin in the high dose progesterone group was seen by naked eye and the scattered pigment deposition was seen in the low dose progesterone group;significant pigment deposition could be found in the ultraviolet group.Compared with the control group,the MDA level of various tissues in the high dose progesterone group was increased,while the SOD level was decreased,serum and skin SOD level in the low dose progesterone group was decreased,the difference was statistically significant（ P 〈0.05）.Serum and skin SOD level in the high dose ultraviolet group was decreased,while the MDA level was increased,the skin SOD level in the low dose ultraviolet group was decreased（ P 〈0.05）,but the difference between the two groups was not statistically significant（ P 〉 0.05）.The melanin grains were heaped up as the small black body under light microscope,which in the progesterone group showed the clutter distribution,the difference between the high dose group and low dose group was significant;which in the ultraviolet group showed the linear dense arrangement and the inter-group had no significant difference. Conclusion Progesterone injection and ultraviolet irradiation all can successfully establish the SD rat chloasma model,and the constructing model effect and stability of ultraviolet irradiation are better.

关 键 词：黄褐斑 紫外线 黄体酮 动物模型 大鼠 sprague-dawley 

分 类 号：R758.42[医药卫生—皮肤病学与性病学]