降扎乡高氡温泉周围居民血浆中8-羟基脱氧鸟苷和硫氧还蛋白还原酶水平的研究  

The level of 8-OHdG and TrxR in the plasma of residents around the radon hot springs in Jiangzha township

作　　者：高延晓 田梅[1] 高刚[1] 刘建香[1] GAO Yanxiao;TIAN Mei;GAO Gang;LIU Jianxiang(Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China)

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088

出　　处：《癌变．畸变．突变》2018年第3期226-229,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:探讨长期多次接触高氡温泉对机体氧化损伤及抗氧化水平的影响。方法:简单随机抽样法选取降扎乡氡温泉周边居民38人作为高氡组,选取阿西茸乡居民39人作为对照组。采用酶联免疫吸附试验(ELISA)检测2组人群血浆中8-羟基脱氧鸟苷(8-OHdG)和硫氧还蛋白还原酶(TrxR)的水平。采用Mann-Whitney U检验比较两组间8-OHdG和TrxR水平的差异。采用问卷调查判断居民对氡的认知情况。结果:高氡组居民外周血血浆中8-0HdG水平明显降低,TrxR水平明显升高(Z=-3.316,-3.773,P均<0.05),分别是对照组的0.571和2.325倍。将氡暴露纳入回归分析,居民氡暴露对8-OHdG和TrxR差异性表达的影响均有统计学意义(P均<0.05)。两组居民对氡、氡对人体健康的影响及减氡措施均缺乏了解。结论:长期多频次接触氡温泉有可能在一定程度上增强机体抗氧化应激能力,降低机体氧化损伤水平。当地居民对氡的健康危害认知严重缺乏,应加强居民的健康教育。OBJECTIVE：To investigate the effects of long-term and repeatedly exposure to radon from hot spring on oxidative damage and antioxidation in the population.METHODS：A simple random sampling method was used to select 38 residents around radon hot spring in Jiangzha township as high radon group,and 39 residents of Axirong township were selected as control group.The levels of 8-hydroxydeoxyguanosine （8-OHdG） and thioredoxin reductase （TrxR） in plasma of the two groups were measured by enzyme-linked immune sorbent assay （ELISA）.The Mann-Whitney U test was used to compare the differences in 8-OHdG and TrxR between the two groups.Questionnaire surveys were conducted on residents＇ cognition of radon.RESULTS：Levels of 8-OHdG in peripheral plasma of residents with high radon group were significantly decreased and levels of TrxR were significantly increased （Z=-3.316,-3.773,P〈0.05）,which were 0.571 and 2.325 times that of the control group respectively.Radon exposure was associated with differential expression of 8-OHdG and TrxR （P〈0.05）.In addition,the two groups of residents lacked knowledge about radon and its impact on human health and about measures to reduce it.CONCLUSION：Long-term and repeatedly exposure to radon from hot springs enhanced body＇s antioxidant capacity to a certain extent,and reduced the level of oxidative damage.Local residents had a serious lack of awareness of radon,and they should be educated about such knowledge.

关 键 词：氡 低剂量电离辐射 8-羟基脱氧鸟苷 硫氧还蛋白还原酶 

分 类 号：R994.6[医药卫生—毒理学]