玉米种子萌发过程内部水分流动规律的低场核磁共振检测  

Detection of internal water flow in germinating corn seeds based on low field nuclear magnetic resonance

作　　者：宋平[1,2,3] 彭宇飞 王桂红 宋鹏[2,3] 王开田 杨涛 Song Ping;Peng Yufei;Wang Guihong;Song Peng;Wang Kaitian;Yang Tao(College of Information and Electrical Engineering, Shenyang Agricultural University, Shenyang 110866, China;Beijing Research Center for Information Technology in Agriculture, Beijing 10097, China;National Research Center of Intelligent Equipment for Agriculture, Beijing 10097, China)

机构地区：[1]沈阳农业大学信息与电气工程学院,沈阳110866 [2]北京农业信息技术研究中心,北京100097 [3]国家农业智能装备工程技术研究中心,北京100097

出　　处：《农业工程学报》2018年第10期274-281,共8页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家重点研发计划:种子精细选别与活性健康检测技术装备研发(2017YFD0701205);国家自然科学基金项目:基于低场核磁共振及其成像技术的水稻种子活力检测与评价方法研究(31701318);辽宁省博士启动基金:基于低场核磁共振及其成像技术的水稻种子活力快速检测方法研究(20170520202)

摘　　要：为研究玉米种子萌发过程中内部水分的流动规律,分析其内部生理代谢状态,该文利用低场核磁共振技术,连续60 h对3个品种玉米种子的吸胀、萌动和发芽3个阶段的萌发过程进行动态监测。分别在萌发时间为0、12、36和60 h这4个典型的萌发时刻,对处于25和31℃的2个恒温萌发环境中的玉米种子进行核磁共振横向弛豫时间信号采集,并通过反演运算得到其横向弛豫时间T_2反演谱。试验结果表明:通过横向弛豫时间可以将玉米种子内部水分划分为结合水(0.1<T_2<10 ms)、半结合水(10<T_2<100 ms)及自由水(100<T_2<1 000 ms)3种水分相态;随着萌发时间的延长,3个品种玉米种子在2个不同温度的萌发环境中,结合水均呈现先迅速增加后逐渐降低的趋势,自由水均反映为先降低后不断增加的态势,而半结合水和总体水分含量则表现为持续增加的现象;外界温度对3种相态水分含量的影响不尽相同,但提高萌发温度可以明显促进玉米种子吸水,同时提高玉米种子的发芽率。本试验快速并直接揭示了玉米种子在萌发过程中的水分分布情况,可为玉米种子萌发过程的机理研究提供重要参考。In order to study the rule of internal water flow in corn seed germination process, the internal physiological metabolism state was analyzed, which changed the traditional research on the germination rate based on quantitative statistics, or considering the change of water from a point of view of time, or to explain the malpractice of the distribution of its internal water by the physiological anatomy of the seed. In this experiment, 3 varieties of corn seeds i.e. Nongda 108, Liaodan 565 and Yuqing 1 were used, which were from the Agricultural College, Shenyang Agricultural University and were the seeds in 2016. The dynamic monitoring of maize seeds at 3 stages of imbibition, germination and sprouting was performed by low field nuclear magnetic resonance （NMR） technique for 60 h, and 160 seeds were selected for each species, every 4 seeds are divided into a group, and 40 parallel test samples were prepared for each species （the sample quality was 1.685 g）. The transverse relaxation time （T2） signal of NMR was collected for maize seeds in 2 constant-temperature germination environments at 25 and 31 ℃, their germination time was 0, 12, 36 and 60 h, each collection was repeated 4 times, the inversion spectrum of the T2was obtained by the inversion.At the same time,the number of germinated seeds in the test sample was counted（taking embryo expansion and skin broken as a criterion）, and then the seed germination rate was calculated. Water was replenished for the experimental samples, which were then continually cultivated in the artificial climate box, and the monitoring of the germination process of maize seeds was realized. The experimental results show that： Through the T2, the internal moisture of maize seed can be divided into 3 water phase states： bound water （0.1〈 T2〈10 ms）, semi bound water （10 〈 T2〈 100 ms） and free water （100 〈 T2〈 1000 ms）; the combination of water molecules and biological macromolecules is closer, which has low kinetic activity, and the amplitu

关 键 词：种子 水分 核磁共振 萌发 

分 类 号：S351.51[农业科学—作物栽培与耕作技术]