南海4个花刺参地理群体的遗传多样性研究  

Genetic Diversity in Four Wild Populations of Sea Cucumber Stichopus monotuberculatus in South China Sea

作　　者：文菁 范嗣刚[2] 李海鹏 胡超群[4] WEN Jing;FAN Sigang;LI Haipeng;HU Chaoqun(Department of Biology, Lingnan Normal University, Zhanjiang 524048, China;South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;Guangzhou University, Guangzhou 510006, China;Key Laboratory of Tropical Marine Bio resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China)

机构地区：[1]岭南师范学院生命科学与技术学院,广东湛江524048 [2]中国水产科学研究院南海水产研究所,广东广州510300 [3]广州大学,广东广州510006 [4]中国科学院南海海洋研究所热带海洋生物资源与生态重点实验室,广东广州510301

出　　处：《水产科学》2018年第3期404-408,共5页Fisheries Science

基　　金：广东特支计划项目(2014TQ01N621);广东省科技计划项目(2017A040405060;2015A030302089;2016A020210136);广东省高等学校优秀青年教师培养计划项目(Yq2014115);广州市市属高校科研项目(1201421216)

摘　　要：在我国南海涠洲岛、临高、琼海和三亚4个地区采集野生花刺参。利用线粒体cox1对这4个花刺参群体83个个体的遗传多样性进行分析,共检测到34个多态位点和23个单倍型。4个群体的核苷酸多样性为0.00128~0.01684,单倍型多样性为0.30719~0.96190。Tajima′s D检验的结果显示,涠洲岛、三亚和琼海群体符合中性进化模型。4个群体的邻接结果显示,涠洲岛先与临高群体聚集在一起,再与三亚聚集,最后与琼海聚集。临高群体和涠洲岛群体之间,三亚群体和琼海群体之间不存在遗传分化,其余群体间有不同程度的遗传分化。4个群体间均存在一定程度的基因流。群体间未形成完全隔阂的地理群体。分子方差分析显示,群体内个体间的遗传变异占87.20%,群体间的遗传变异占12.80%。从单倍型邻接树和单倍型网络关系图中未发现有地理谱系结构的单倍型。A 655 base-pair fragment of mitochondrial cox1 gene was used for analysis of genetic diversity in four wild populations including Lingao（LG）,Qionghai（QH）,Sanya（SY）and Weizhou island（WZ）of sea cucumber Stichopus monotuberculatus.Thirty-four polymorphic sites were found in cox1 gene and 23 haplotypes were detected in 83 samples.Overall,nucleotide diversity（π）was ranged from 0.00128 to0.01684 and haplotype diversity（Hd）was varied from 0.30719 to 0.96190.Tajima′s D analysis revealed that WZ,SY and QH populations were satisfied with neutral theory of molecular evolution,and the neighbour-joining（N-J）tree of these four populations indicated that the WZ population was clustered with the LG population first,and with SY and QH subsequently.The analysis of Fst showed that there were different levels of population differentiation among these four populations except between WZ and LG and between QH and SY.There was no independent geographic population due to different levels of gene flow among these four populations.The AMOVA analysis indicated that 87.20% of genetic diversity was derived from individuals in the four populations and 12.80% of genetic diversity from segregation population.The analysis of N-J tree and network of 23 haplotypes indicated that there were no geographic haplotype.The findings provided genetic background of sea cucumber S.monotuberculatus for conservation and management.

关 键 词：花刺参 群体 遗传多样性 单倍型 基因流 

分 类 号：S968.9[农业科学—水产养殖]