2型糖尿病db/db小鼠肾脏动态病理特点  

Dynamic pathological characteristics of kidney in db/db mice with type 2 diabetes mellitus

作　　者：孙竹梅 梁伟时 康佳丽 孟徐兵 王建行[2] 韩淑英 SUN Zhumei;LIANG Weishi;KANG Jiali;MENG Xubing;WANG Jianxing;HAN Shuying(School of Clinical Medicine,North ChinaUniversity of Science andTechnology,Tangshan 063000,China;J i tang College,North ChinaUniversity of Science andTechnology,Tangshan 063000,China;C^ollege of Basic Medical Sciences,North ChinaUniversity of Science andTechnology,Tangshan 063000,China)

机构地区：[1]华北理工大学临床医学院,河北唐山063000 [2]华北理工大学冀唐学院,河北唐山063000 [3]华北理工大学基础医学院,河北唐山063000

出　　处：《吉林大学学报（医学版）》2018年第3期499-503,695,共6页Journal of Jilin University：Medicine Edition

基　　金：河北省中医药管理局科研项目资助课题(QN2017083)

摘　　要：目的:探讨2型糖尿病db/db小鼠肾脏动态病理特点,阐明其糖尿病肾病的发生发展机制,为糖尿病肾脏并发症的研究提供实验依据。方法:选取SPF级7~8周龄雄性db/db小鼠(模型组)和同龄雄性db/m小鼠(正常组)各16只,分别于8、16和32周龄时检测2组小鼠体质量和空腹血糖(FBG)水平,每组各处死小鼠8只,取肾组织行HE和Masson染色观察其病理形态表现,电镜下观察其超微结构。结果:与正常组比较,模型组小鼠8、16和32周龄时体质量增大(P<0.01),FBG水平明显升高(P<0.01),双肾指数明显降低(P<0.01);32周时模型组小鼠双肾指数明显低于16周时(P<0.05)。HE和Masson染色,16周龄时模型组小鼠肾组织可见明显病理改变,主要是肾小球体积增大和肾小管上皮细胞明显水肿;32周龄时病变明显加重,肾组织中可见大量蓝染胶原物质。电镜观察,16周龄时模型组小鼠肾组织以肾小球基底增厚、足突融合、肾小管上皮细胞线粒体数目减少及形态肿胀为主;32周龄时病变明显加重,可见大量胶原纤维。结论:16周龄糖尿病db/db小鼠肾脏有明显病理改变,主要是肾小球基底增厚、足突融合,32周龄时小鼠肾组织呈明显纤维化。Objective：To investigate the dynamic pathological characteristics of kidney in the db/db mice with type 2 diabetes mellitus and to clarify the pathogenesis and development mechanism of diabetic nephropathy,and to provide the experimental evidence for the further study on diabetic nephropathy.Methods：The male SPF db/db mice aged 7-8 weeks were selected as model group（n=16）and the db/m mice with the same ages were selected as control group（n=16）.The body weights and levels of fasting blood glucose（FBG）of the mice were detected after 8,16,and 32 weeks.Then eight mice in each group were sacrificed and the kidney tissue was dissected at 8,16 and 32 weeks.HE and Masson staining were used to observe the pathomorphology of the kidney tissue;the ultrastructures of kidney tissue were observed under electron microscope.Results：Compared with normal group,the body weights of the mice in model group at 8,16 and 32 weeks were significantly increased（P〈0.01）,the FBG levels were significantly increased（P〈0.01）,and the double kidney indexes were significantly decreased（P〈0.01）;the double kidney index of the mice in model group at 32 weeks was lower than that at 16 weeks（P〈0.05）.The HE and Masson staining results showed that the morphological changes of kidney tissue of the mice at16 weeks were significant,the glomerular volume was expansion and the kidney tubular epithelial cells were edema,which turned serious at 32 weeks.A large number of blue dye substances in the kidney tissue was found.Under electron microscope,the glomerular basement membranes got thickened,the foot processes got fused and the number of mitochondria in kidney tubular epithelial cells was reduced and swell was found in the mice in model group at 16 weeks;the lesions in the kidney tissue were serious at 32 weeks,and the collagenous fiber was visible at32 weeks.Conclusion：The pathological changes in the kidney tissue of the db/db mice at 16 weeks are significant,such as glomerular basement thickening and footwork fusi

关 键 词：2型糖尿病 DB/DB小鼠 空腹血糖 HE染色 MASSON染色 

分 类 号：R587.1[医药卫生—内分泌]