金银花多糖的提取纯化及抗病毒活性研究  

Extraction and purification of the Lonicera japonica polysaccharide and its antiviral activity in vitro

作　　者：王剑[1] 侯林[1] 陈亚乔 张成华[1] 耿巧玉 范路路[1] 刘金安[1] 田景振[1] WANG Jian, HOU Lin, CHEN Ya-qiao, ZHANG Cheng-hua, GENG Qiao-yu, FAN Lu-lu, LIU Jin-an, TIAN Jing-zhen(School of Pharmacy, Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, Chin)

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《中国医院药学杂志》2018年第8期810-812,共3页Chinese Journal of Hospital Pharmacy

基　　金：国家科技重大专项子课题;治疗温病的中药新药研发(编号:2014ZX09509001);山东省高等学校中医药抗病毒协同创新中心课题;淡竹叶;鸭跖草等10味中药的抗病毒谱研究(编号:XTCX2014C01-01);山东省重点研发计划(重大关键技术及重点产业关键技术)-经方中成药葛根汤颗粒等二次开发研究(编号:2016CYJS08A01-8)

摘　　要：目的:提取纯化金银花多糖,并研究金银花多糖的体外抗病毒活性。方法:采用醇沉法提取多糖并用酶法纯化多糖,运用细胞培养技术,对纯化后的多糖进行体外抗病毒实验,以MTT染色法检测细胞活性,结合CPE法研究金银花多糖对单纯疱疹病毒(HSV-1)、柯萨奇病毒B5(COX-B5)、柯萨奇病毒B3(COX-B3)和肠道病毒71型(EV71)的抑制作用。结果:多糖纯化后含量为54.04%,金银花多糖对COX-B5、EV71、COX-B3、HSV-1的治疗指数分别为4.84,63.85,4.77,51.25。结论:金银花多糖具有体外抗HSV-1、EV71、COX-B5、COX-B3的活性,为临床治疗提供理论依据。OBJECTIVE To extract and purify the Lonicera japonica polysaccharide and study its antiviral activity in vitro.METHODS The Lonicera japonica polysaccharide was extracted by alcohol precipitation method and purified by enzymatic method.Taking the experiment in vitro antivirus of cell culture,CPE and MTT methods were adopted to investigate the inhibitory effects of the Lonicera japonica polysaccharide on several common viruses such as Enterovirus 71（EV71）,Coxsackievirus B5（COX-B5）,Coxsackievirus B3（COX-B3）and Herpes simplex virus typeⅠ（HSV-1）.RESULTS The content of polysaccharide was 54.04%.The therapeutic indexes（TI）of COX-B5,EV71,COX-B3 and HSV-1 were 4.84,63.85,4.77 and51.25,respectively.CONCLUSION Lonicera japonica polysaccharide has antiviral activity against COX-B5,EV71,COX-B3 and HSV-1,and provides a theoretical basis for clinical application.

关 键 词：金银花多糖 抗病毒 EV71 HSV-1 COX-B5 COX-B3 

分 类 号：R969.1[医药卫生—药理学]