脊髓缺血性损伤时脑源性神经营养因子、丝氨酸/苏氨酸蛋白激酶ε蛋白和mRNA含量的变化及远程缺血预处理干预作用  

Brain-derived neurotrophic factor,Serine/threonine protein kinase Cε,change in mRNA content and remote ischemic preconditioning intervention in rabbit models with spinal cord ischemic reperfusion injury

作　　者：余奇劲[1] 杨云朝[1] YU Qijing, YANG Yunzhao.(Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan 430060, Chin)

机构地区：[1]武汉大学人民医院麻醉科,430060

出　　处：《临床外科杂志》2018年第4期307-309,共3页Journal of Clinical Surgery

基　　金：湖北省卫生计生委科研资助项目(WJ2015MB023)

摘　　要：目的探讨远程缺血预处理(RIPC)对兔脊髓缺血再灌注损伤(SCIRI)时脑源性神经营养因子(BDNF)、丝氨酸/苏氨酸蛋白激酶(PKC)ε蛋白和mRNA含量的影响及其意义。方法日本大耳白兔36只,随机均分为假手术组(S组)、缺血性损伤组(IR组)、IR+RIPC组。每组6只动物分别于再灌注第2天和第5天处死。S组不阻断腹主动脉,IR组和IR+RIPC组夹闭腹主动脉30分钟建立SCIRI模型,IR+RIPC组于主动脉阻断前1小时实施RIPC。3组动物在再灌注第2天和第5天行后肢神经功能评分后处死并取脊髓组织L3-L5段,评估病理学变化;用Western blotting和RT-PCR法分别检测脊髓组织BDNF、PKCε蛋白及其mRNA含量。结果同一时间点,与IR组相比,IR+RIPC组后肢神经功能评分和脊髓组织病理切片分级明显改善(P<0.05),脊髓组织BDNF、PKCε蛋白及mRNA表达均明显升高(P<0.05)。结论 RIPC对兔SCIRI有一定的防治作用,其作用机制与RIPC激活了PKCε/PKC信号通路,进而上调脊髓损伤区域BDNF蛋白的表达有关。Objective To investigate the effects of remote ischemic preconditioning（ RIPC） on the expression of brain-derived neurotrophic factor（ BDNF）,Serine/threonine protein kinase Cε（ PKCε） in spinal cord tissues and change in mRNA content after spinal cord ischemic reperfusion injury（ SCIRI）.Methods A total of 36 cases of Japanese white rabbits were randomly divided into sham（ group S）,ischemic reperfusion injury（ group IR） and group IR ＋ RIPC（ 12 rabbits in each group）. Each group was further divided into two sub-groups according to time points after reperfusion（ 2 and 5 days）,six rabbits of each group were sacrificed at each time point. In group S,abdominal aorta were only separated and exposed and were not camped. In group IR and group IR ＋ RIPC,the abdominal aorta were camped for 30 min,and the SCIRI models were established. In group IR ＋ RIPC,RIPC was performed 1 h before aortic calmping. Hind-limb neurological function of each group was evaluated using Tarlov Scale at 2,5 d after surgery,then rabbits were sacrificed,and L4-L6 spinal cord segments were taken. Pathological change in spinal cord tissues were observed,the protein and mRNA expression of BDNF and PKCε were detected by Western blotting analysis and PT-PCR. Results In comparison with group IR,hind-limb neurologic function scores at the same time point were significantly higher（ P〈 0. 05）,and the protein and mRNA expression of BDNF and PKCε were significant increased in group IR ＋ RIPC（ P〈 0. 05）. Conclusion RIPC has an important role in prevention and treatment of SCIRI in rabbits. The mechanisms may be that RIPC activates the PKCε/PKC signaling pathway and up-regulates the expression of BDNF and PKCε in spinal cord tissues after spinal cord injury.

关 键 词：远程缺血预处理 脊髓 缺血再灌注损伤 脑源性神经营养因子 丝氨酸/苏氨酸蛋白激酶ε 

分 类 号：R651.2[医药卫生—外科学]