光照和培养基对内生真菌棘豆链格孢Alternaria oxytropis苦马豆素含量的影响  

Influence of light and medium on the content of swainsonine in the endophytic fungus Alternaria oxytropis

作　　者：李欣 卢萍[1] 吕桂芬[1] 袁博 LI Xin;LU Ping;LU Gui-fen;YUAN Bo(College of Life Science and Technology, Inner Mongolia Normal University, Hohhot, Inner Mongolia 010022, China)

机构地区：[1]内蒙古师范大学生命科学与技术学院,内蒙古呼和浩特01002

出　　处：《菌物学报》2018年第5期633-639,共7页Mycosystema

基　　金：国家自然科学基金(31460235)~~

摘　　要：以小花棘豆内生真菌棘豆链格孢Alternaria oxytropis野生株OW7.8及其酵母氨酸还原酶基因缺失突变株M1为材料,研究它们在不同培养基上和不同光照下菌落生长速率与苦马豆素含量。结果表明:OW7.8在胡萝卜葡萄糖琼脂培养基上暗培养时生长速度最快,为(2.57±0.17)mm/d,而M1则在马铃薯葡萄糖琼脂培养基上暗培养时生长速度最快,为(4.93±0.10mm)/d。不同培养基和光照条件下,相同菌株苦马豆素的含量差异不显著(P>0.05);不同菌株在相同培养条件下其苦马豆素含量差异显著(P<0.05)。The wild type strains OW7.8 and saccharopine reductase gene disruption mutant M1 of the endophytic fungus Alternaria oxytropis from Oxytropis glabra were selected as materials. Colony growth and swainsonine content were studied in different culture media and light conditions. The results showed that under darkness culture condition the fastest growth rate in OW7.8 reached（2.57±0.17）mm/d in carrot dextrose agar, while that in M1 reached（4.93±0.10）mm/d in potato dextrose agar. There was no significant difference in the content of swainsonine under different medium and light conditions in the same strain, either OW7.8 or M1（P〉0.05）. However, there was significant difference in the content of swainsonine between OW7.8 and M1 under the same culture conditions（P〈0.05）.

关 键 词：小花棘豆 培养条件 苦马豆素 

分 类 号：Q936[生物学—微生物学]