Additive Effects of Water-Soluble Propolis(Greit 120)and Human Interferon Alfa(HuIFN-αN3)against Influenza Viruses in Vitro  

作　　者：Bratko Filipic Lidija Gradisnik Adriana Pereyra Rok Kopino Klemen Rihar Eva Ruzic-Sabljic Snezana Kramar Damir Dermie Eugen Soos Nicola Volpi Alfredo Fachini and Hrvoje Mazija 

机构地区：[1]Croatian Institute of Experimental and Translation Oncology,Koledinecka 3,Zagreb 10040,Croatia [2]Institute of Biomedical Sciences,Medical Faculty of Maribor,Taborska 8,Maribor 2000,Slovenia [3]Medex D.o.o.,Linhartova 49b,Ljubljana 1000,Slovenia [4]Chengdujska 4,Ljubljana 1000,Slovenia [5]Institute of Microbiology and Immunology,Medical Faculty,Zaloska 4,Ljubljana 1105,Slovenia [6]Division of Molecular Biology,Ruaer Boskovic Institute,Bijenicka 54,Zagreb 10000,Croatia [7]Trg Sv.Ivana 5,Klostar Ivanic 10312,Croatia [8]Department of Life Sciences,University of Modena&Reggio Emilia,Modena 41125,Italy [9]BNatural,Via Gran Sasso 33,Corbetta-MI 20011,Italy

出　　处：《Journal of Agricultural Science and Technology(A)》2018年第1期36-47,共12页农业科学与技术（A）

摘　　要：Influenza virus affects the respiratory tract in humans causing a range of distinct manifestations including fever, nasal secretions, cough, headaches, muscle pain and pneumonia, which could become violent and severe. It was found that influenza A viruses remain resistant to amantadine and rimantadin with high level of oseltamvir resistance. Therefore, there is a need for constant improvement of drugs active against resistant influenza viruses. Propolis has anti-influenza activity both in vitro and in vivo. Human leukocyte interferon （HuIFN-αN3） is a multi-subtype protein that displays an antiviral activity against influenza virus. In this study we elucidated the anti-influenza activity of the mixes of water-soluble propolis （WSP） （Greit 120） and HuIFN-αN3 at different ratios. Greit 120 polyphenols, total phenol acids and bioflavonoid were characterized by HPLC-UV-ESI-MS504971 and HuIFN-αN3 by reverse-phase high-performance liquid chromatography （RP-HPLC）. Influenza A and B viruses were separately added to the LLC-MK2 cells treated with WSP （Greit 120） and HuIFN-αN3 alone or in proportions 1：1, 1：2 and 2：1. Plates were incubated and cytopathic effect was determined. The best results （ID50） were obtained with the mix of 10% WSP and HuIFN-αN3 in proportion 1：2, showing ID50 at 12 ± 0.2 μg/mL and 19 ± 0.6 μg/mL for influenza A and B viruses, respectively. When comparing anti-influenza activity of WSP （Greit 120）/HuIFN-αN3 with that of ribavirin, it was found that 1：2 was the optimal ratio for WSP （Greit 120）/HuIFN-αN3 （0.5 and 0.6 for influenza A and B, respectively）. This new formulation of WSP （Greit 120） and HuIFN-αN3, showing better anti-Influenza activity, will definitely improve its application in flu infections.

关 键 词：Water-soluble propolis Greit 120 HuIFN-αN3 anti-influenza activity ribavirin ID_(50) 

分 类 号：S858.216.3[农业科学—临床兽医学] Q78[农业科学—兽医学]