西红花水提物对实验性糖尿病小鼠血糖水平的影响  

Effect of saffron aqueous extract on the level of blood glucose in experimental diabetes mice

作　　者：江石平[1] 沈倩 陆雨[1] 晏永球 童应鹏[1] 王平[1] JIANG Shi-ping;SHEN Qian;LU Yu;YAN Yong-qiu;TONG Ying-peng;WANG Ping(College of Pharmaceutical Sciences,Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]浙江工业大学药学院,杭州310014

出　　处：《中国应用生理学杂志》2018年第2期173-176,共4页Chinese Journal of Applied Physiology

基　　金：浙江省特色中药材西红花深加工关键技术与系列功能性食品开发(2015C02032)

摘　　要：目的:观察西红花水提物对链脲佐菌素(STZ)诱导的糖尿病小鼠血糖、血脂及胰腺组织的影响。方法:采用STZ(60 mg/kg)连续2 d腹腔注射建立糖尿病小鼠模型。将造模成功后的小鼠随机分为3组(n=10):糖尿病模型(DM)组、西红花水提物(SE)组、阳性对照二甲双胍(MH)组。另取10只正常小鼠设为正常对照(NC)组。给药组每天灌胃1次,连续6周,模型组和正常对照组灌胃生理盐水。给药期间每周测定小鼠进食量、饮水量及体重,给药6周后测定空腹血糖(FBG)、口服糖耐量(OGTT)、糖化血清蛋白(GSP)、血清胰岛素(INS)和血脂等指标的变化情况;HE染色观察胰腺组织病理变化。结果:与NC组相比,DM组进食量、饮水量、线下曲线面积、FBG、GSP以及血脂中的总胆固醇(TC)均显著升高,空腹体重、血清胰岛素(INS)及高密度脂蛋白胆固醇(HDL-c)均显著降低;与DM组相比,SE组小鼠饮水量、FBG、线下曲线面积、TC显著降低,HDL-c以及INS显著升高。病理学显示DM组胰岛结构破坏、胰岛细胞数量明显减少、胰岛血管增生、形态不规则等变化,SE能明显修复受损胰腺组织。结论:SE对链脲佐菌素诱导的糖尿病小鼠有一定降血糖、降血脂作用,可以有效改善胰腺病变的情况,提示西红花可能用于糖尿病的防治。Objective： To investigate the effects of saffron aqueous extract（ SE） on blood glucose,lipid and pancreatic tissue in streptozocin-induced diabetes mice. Methods： Diabetes mellitus mice were established by intraperitoneal injection of streptozocin（ 60 mg/kg） for two consecutive days. The 30 well-established diabetes mice were randomly divided into three groups（ n = 10） ： diabetic mellitus（ DM） group,SE treated（ SE） group and positive control（ metformin hydrochloride,MH） group. Another ten normal mice were selected as normal control（ NC） group. The mice in SE and MH groups were intragastrically administered with SE 100 mg/kg or MH 100 mg/kg once a day for 6 weeks,mice in DM and NC were given normal saline. The amount of food-intake,water consumption and body weight were measured weekly,the changes of the indicators including fasting blood glucose（ FBG）,oral glucose-tolerance test（ OGTT）,glycated serum protein（ GSP）,insulin（ INS） and blood lipid were determined after 6 weeks of continuous administration. The pathologic changes in the pancreas tissues were detected by HE staining. Results： Compared with the normal control group,the amount of food-intake,water consumption,area under the curve,FBG,GSP,and total cholesterol（ TC） were significantly increased,while fasting weight,INS and high density lipoprotein cholesterol（ HDL-c） were dramatically decreased in DM group. Compared with DM group,the water consumption,FBG,area under the curve and TC in SE group were starkly declined with a notable elevation of HDL-c and INS. In addition,the biopsy from DM mice showed the structure of pancreas islet was destroyed and reduced,and vascular proliferation with irregular shape. The damaged pancreas was obviously repaired by giving SE. Conclusion： The saffron aqueous extract had efficacy on blood glucose and blood lipids reduction,improvement on damaged pancreas in streptozocin-induced diabetic mice,which suggested that saffron could be used for the treatment in diabet

关 键 词：西红花 糖尿病 血糖 血脂 小鼠 

分 类 号：R285.5[医药卫生—中药学]