淡水溺死大鼠肺组织与血清中IL-1α、IL-1β和IL-13mRNA表达  

Expressions of IL-1α, IL-1β and IL-13 m RNA in Lung Tissue and Serum of Rats Drown in Fresh Water

作　　者：姜美玲 刘志杰 潘静 路健[2] 梁新华[2] JIANG Mei-ling;LIU Zhi-jie;PAN Jingg;LU Jian;LIANG Xin-hua(Mingzheng Forensic Identification Centre of Jilin, Jilin 132011, China;College of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China;Linxi County Public Security Bureau of Chifeng City, Chifeng 025250, China)

机构地区：[1]吉林鸣正司法鉴定中心,吉林吉林132011 [2]山西医科大学法医学院,山西太原030001 [3]赤峰市林西县公安局,内蒙古赤峰025250

出　　处：《法医学杂志》2018年第2期111-113,119,共4页Journal of Forensic Medicine

摘　　要：目的检测溺死大鼠肺组织和血清中白细胞介素(interleukin,IL)-1α、IL-1β、IL-13 m RNA的变化,探讨其在法医学溺死诊断鉴定中的潜在价值。方法 18只SD大鼠随机分为溺死组、空白对照组和心肌梗死组(对照)。分别收集各组大鼠右心室血清及右肺下叶、心肌,应用Taq Man探针法检测肺组织及右心室血清IL-1α、IL-1β、IL-13 m RNA的表达情况。结果与空白对照组及心肌梗死组比较,溺死组大鼠肺组织IL-1α、IL-1β及IL-13 m RNA表达差异均无统计学意义(P>0.05),右心室血清IL-1β、IL-13 m RNA表达升高(P<0.05)。空白对照组与心肌梗死组比较,血清中IL-1α、IL-1β及IL-13 m RNA表达差异均无统计学意义(P>0.05)。结论细胞因子IL-1β及IL-13 m RNA在溺死后大鼠右心室血清中表达量均有改变,表明其变化与溺死密切相关。Objective To detect the changes of（interleukin, IL）-1α, IL-1β and IL-13 m RNA in lung tissue and serum of drown rats, and to explore the potential value for the diagnosis of drowning in forensic practice. Methods Eighteen SD rats were randomly divided into drowning group, blank control group and myocardial infarction group（as control group）. The serum of right ventricular, the inferior lobe of right lung and the myocardium were taken from the rats in different groups. The expressions of IL-1α, IL-1β and IL-13 m RNA in the lung tissue and the serum of right ventricular were detected by Taq Man probe method. Results The expression differences of IL-1α, IL-1β and IL-13 m RNA in lung tissue between drowning group and blank control group, myocardial infarction group were not statistically significant（P〈0.05）. The expression of IL-1β and IL-13 m RNA in serum of right ventricular increased（P〈0.05）. The expression differences of IL-1α, IL-1β and IL-13 m RNA in serum between blank control group and myocardial infarction group were not statistically significant（P〉0.05）. Conclusion The changes of cytokines IL-1β and IL-13 m RNA in the serum of right ventricular of drown rats are statistical significance, which are highly correlated with drowning.

关 键 词：法医病理学 溺水 淡水 白细胞介素-1Α 白细胞介素-1Β 白细胞介素-13 大鼠 

分 类 号：DF795.1[医药卫生—法医学]