联苯胺试验对血痕DNA检验的影响  

Effect of Benzidine Test on DNA Analysis of Bloodstain

作　　者：金明[1] 巴华杰[1] 朱爱华[1] 马骏[1] 石津玮 刘亚楠[2] 林子清[3] JIN Ming;BA Hua-jie;ZHU Ai-hua;MA Jun;SHI Jin-wei;LIU Ya-nan;LIN Zi-qing(Institute of Forensic Science,Changzhou Public Security Bureau, Changzhou 213003, China;Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science,Shanghai Public Security Bureau, Shanghai 200083, China;Department of Forensic Medicine, China Criminal Police College,Shenyang 110035, China)

机构地区：[1]常州市公安局物证鉴定所,江苏常州213003 [2]上海市公安局物证鉴定中心法医物证学现场应用技术公安部重点实验室上海市现场物证重点实验室,上海200083 [3]中国刑警学院法医学系,辽宁沈阳110035

出　　处：《法医学杂志》2018年第2期157-160,共4页Journal of Forensic Medicine

摘　　要：目的探讨联苯胺试验及相关试剂对血痕DNA检验的影响。方法制作含1μL静脉血的滤纸血痕970份,其中10份为对照样本,960份经联苯胺试验及相关试剂分别处理后,采用Chelex-100法和硅珠法提取DNA,Amp F詛STR^(TM)Identifiler^(TM)Plus PCR扩增试剂盒进行复合扩增,对比各组STR分型结果。结果联苯胺试验后立即提取DNA,硅珠法的STR基因座检出数为(3.80±1.34)个,而Chelex-100法均未获得STR分型结果;联苯胺试验后干燥处理,硅珠法有13例(21.7%)获得全部STR基因座分型结果,且STR基因座检出数[(12.90±1.49)个]远高于Chelex-100法[(4.70±1.96)个](P<0.05);加入冰醋酸后立即提取DNA,硅珠法的STR基因座检出数为(9.40±2.09)个,而Chelex-100法均未获得STR分型结果;只加冰醋酸后干燥处理以及只加四甲基联苯胺乙醇饱和溶液或3%过氧化氢溶液,两种方法均获得完整15个STR基因座分型结果。结论联苯胺试验对血痕的后续DNA检验有很大的影响,Chelex-100法不适合联苯胺试验后血痕的DNA检验,联苯胺试验后干燥处理及采用硅珠纯化的方法可有效提升联苯胺试验后血痕的STR基因座检出数。Objective To explore the effect of benzidine test and related reagents on DNA analysis of bloodstain.Methods A total of 970 bloodstain filter paper samples with 1μL venous blood were collected,and 10 of them acted as control samples.After benzidine test and related reagent processing,DNA of960 samples was extracted by Chelex-100 and silica bead methods and then multiplex amplified by Amp FlSTR^TMIdentifiler^TM Plus PCR kits.The results of STR typing were compared between different groups.Results DNA were extracted immediately after benzidine test.Totally STR loci（3.80±1.34）were detected by silica bead method,while no STR loci were obtained by Chelex-100 method.Thirteen samples（21.7%）with whole STR typing results were obtained by drying after benzidine test,and the STR locus number（12.90±1.49）which obtained by silica bead method was much higher than by Chelex-100method（4.70±1.96）（P〈0.05）.When DNA was extracted immediately after the addition of glacial acetic acid,the STR locus number was（9.40±2.09）by silica bead method,but no STR typing result was obtained by Chelex-100 method.All 15 STR loci could be obtained by only adding glacial acetic acid after drying and only adding tetramethylbenzidine alcoholization liquid or 3%hydrogen peroxide liquid.Conclusion Benzidine test has significant influence on DNA analysis of bloodstain.The Chelex-100 method is not suitable for the DNA extraction of bloodstain after benzidine test.Drying after benzidine test and silica bead methods can effectively enhance the STR locus number of bloodstain.

关 键 词：法医遗传学 联苯胺试验 血迹 DNA提取 STR分型 

分 类 号：DF795.2[医药卫生—法医学]