过表达拟南芥AtCIPK 23、AtAKT 1和AtCBL 1基因棉花苗期耐低钾的差异及机制研究  

The Difference and Mechanism in Tolerance to Low-Potassium among Cotton Seedlings Over-Expressed Arabidopsis AtCIPK 23, AtAKT 1 and AtCBL 1 Genes

作　　者：张斌 刘记 张朝军[1] 孔德培 王鹏 杨召恩 李付广[1] 张雪妍[1] Zhang Bin;Liu Ji;Zhang Chaojun;Kong Depei;Wang Peng;Yang Zhao＇ en;Li Fuguang;Zhang Xueyan(State Key Laboratory of Cotton Biology,Institute of Cotton Research of Chinese Academy of Agricultural Sciences,Anyang,Henan 455000,Chin)

机构地区：[1]中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳455000

出　　处：《棉花学报》2018年第3期205-214,共10页Cotton Science

基　　金：中央级公益性科研院所基本科研业务费专项(1610162016011);国家重点研发计划-棉花品质;抗逆等重要性状的功能基因组与调控网络(2016YFD0101006)

摘　　要：【目的】评价过表达拟南芥At CIPK 23、At AKT 1和At CBL 1基因棉花苗期耐低钾能力的差异,为棉花钾高效品种培育提供理论依据。【方法】通过室内水培法,测定比较了转基因棉花和中棉所24(CCRI 24)在适钾、低钾条件下苗期的长势、干物质质量、钾浓度和钾累积量等指标,并从根系形态、钾利用指数、钾转运能力和受胁迫程度等方面综合分析了其可能的生理机制。【结果】适钾条件下,转基因棉花和中棉所24长势基本一致,无显著差异。低钾胁迫时,过表达At AKT 1基因棉花幼苗总根长、根表面积和根体积约为中棉所24的3倍,整株干物质质量是中棉所24的1.66倍,具有明显的生长优势,并且钾转运能力强、钾利用指数高,受胁迫程度明显降低;而过表达拟南芥At CIPK 23和At CBL 1基因棉花幼苗的各项指标与中棉所24相当,无显著差异。【结论】在棉花中过表达拟南芥钾离子通道蛋白基因At AKT 1能显著提升棉花本身的耐低钾能力。[Objective] To provide theoretical basis for potassium efficient cotton breeding, this work aimed to evaluate the influ- ence of low- potassium on transgenic cotton seedlings over-expressing Arabidopsis thaliana ＂s AtCIPK 23, AtAKT 1 and AtCBL 1 genes, respectively. [Method] Hydroponics was used to explore the growth vigor, dry weight, potassium concentration and ac- cumulation of transgenic cotton and CCRI 24 seedlings under low- and optimum potassium conditions. Furthermore, the root morphology, potassium utilization index, potassium transport ability and the severity to damage were comprehensively analyzed to uncover the possible physiological mechanisms. [Result] Under the optimum conditions of potassium, the transgenic plants and control grew- well and showed no significant difference. However, with low- potassium stress, the dry weight of plants over-expressing AtAKT 1 gene was 1.66 times ofCCR124. Its total root length, root surface area and volume were about 3 times than those of CCRI 24. The AtAKT 1 plants also displayed obvious growth advantage, higher potassium utilization index and transport ability, and the severity to damage was lower. Plants over-expressing AtC1PK 23 and AtCBL 1 genes, however, were almost the same as the CCRI 24. [Conclusion] This study revealed that the ability of cotton＇s resistance to low- potassium was significantly improved by over-expression of AtAKT 1 gene, an exogenous potassium ion channel protein gene from Arabidop- sis thaliaaa

关 键 词：中棉所24 苗期 耐低钾 钾利用 胁迫 

分 类 号：S562.01[农业科学—作物学]