表达猪流行性腹泻病毒保护性抗原重组乳酸杆菌的构建及鉴定  

Construction and Identification of Recombinant Lactobacillus casei Expressing Protective Antigens of Porcine Epidemic Diarrhea Virus

作　　者：孙玉章 许运斌[2] 王宏华 孙明军[3] 蒋贻海 Sun Yuzhang;Xu Yunbin;Wang Honghua;Sun Mingjun;Jiang Yihai(Qingdao Vland Biotech Group Co. Ltd,National Research Center for Animal Health Products,Qingdao,Shandong266000,China;Zunyi Medical University,Guizhou Provincial College-based Key Lab of Infectious Disease ＆ Biosafety,Zunyi,Guizhou563000,China;China Animal Health ＆ Epidemiology Center,Qingdao,Shandong266032,China)

机构地区：[1]青岛蔚蓝生物股份有限公司国家动物用保健品工程技术研究中心,山东青岛266000 [2]遵义医学院贵州省传染病与生物安全特色重点实验室,贵州遵义563000 [3]中国动物卫生与流行病学中心,山东青岛266032

出　　处：《中国动物检疫》2018年第6期94-97,共4页China Animal Health Inspection

基　　金：国家重点研发计划专项(2016YFD0501308)

摘　　要：为开发一种安全有效的新型猪流行性腹泻口服疫苗,将猪流行性腹泻病毒核心保护性抗原基因(COE)插入到穿梭表达载体,并电转化至乳酸杆菌基因工程菌株内,进行诱导培养,通过Western blot能够检测到大小约27 KD的蛋白表达。该重组乳酸杆菌的构建为动物体内免疫试验的开展和新型黏膜免疫疫苗的研发奠定了基础。In order to develop a safe and effective oral vaccine for Porcine epidemic diarrhea（PED）,the core neutralizing epitope（COE）gene of PEDV was cloned and inserted into the shuttle expression vector. Then the vector was electric-transformed into the genetically engineered strain of Lactobacillus casei. After bacteria culture and protein induction,a stripe of about 27 KD was detected by western blot. As a result,the develop of recombinant Lactobacillus laid foundation for further study of animal immunological experiments in vivo and novel PED mucosal vaccine.

关 键 词：猪流行性腹泻病毒 重组乳酸杆菌 COE基因 黏膜免疫 口服疫苗 

分 类 号：S851.3[农业科学—预防兽医学]