Low Shear Stress Regulating Autophagy Mediated by the p38 Mitogen Activated Protein Kinase and p53 Pathways in Human Umbilical Vein Endothelial Cells  

作　　者：Hui-Zhen Liu Li Li Shao-Liang Chen Jian-Rui Wei Jun-Xia Zhang Jia Liu Jie-Wen Guo Xin-Liang Qu Peng Chu 

机构地区：[1]Department of Cardiology, Guangzhou Red Cross Hospital, Jinan University affiliated Guangzhou Red Cross Hospital, Guangzhou, Guangdong 510000, China [2]Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China

出　　处：《Chinese Medical Journal》2018年第9期1132-1133,共2页中华医学杂志（英文版）

基　　金：This study was supported by grants from the Natural Science Foundation of Guangdong Province （No. 2016A030313430）, the Scientific and Technological Program of Guangzhou City （No. 201510010024）, and the Natural Science Foundation of Guangdong Province （No. 2014A030310049）.

摘　　要：To the Editor：Autophagy is reported to play a critical role in low shear stress （LSS）-induced endothelial cell injury and the formation of atherosclerotic plaques.[1] However,the corresponding mechanisms remain unclear.[2-8] This study was to investigate the changes and mechanism of LSS-induced autophagy in human umbilical vein endothelial cells （HUVECs）.HUVECs were treated with LSS of 5 dyn/cm2 for 0,5,15,30,and 60 min in a parallel plate flow chamber system.Light chain （LC） Ⅱ,LC3 Ⅰ,and p62,p38 mitogen-activated protein kinase （MAPK） and their protein of phosphorylation of p38 （p-p38） were detected with Western blot analysis.The protein levels of p-p53 （ser15） and their distribution were detected by immunofluorescence （IF）.To the Editor：Autophagy is reported to play a critical role in low shear stress （LSS）-induced endothelial cell injury and the formation of atherosclerotic plaques.[1] However,the corresponding mechanisms remain unclear.[2-8] This study was to investigate the changes and mechanism of LSS-induced autophagy in human umbilical vein endothelial cells （HUVECs）.HUVECs were treated with LSS of 5 dyn/cm2 for 0,5,15,30,and 60 min in a parallel plate flow chamber system.Light chain （LC） Ⅱ,LC3 Ⅰ,and p62,p38 mitogen-activated protein kinase （MAPK） and their protein of phosphorylation of p38 （p-p38） were detected with Western blot analysis.The protein levels of p-p53 （ser15） and their distribution were detected by immunofluorescence （IF）.

分 类 号：R54[医药卫生—心血管疾病]