艾灸对瞬时受体电位香草酸亚型1基因敲除小鼠局部皮肤中肿瘤坏死因子-α表达的影响  

Moxibustion-induced Regional Changes of Histological Structure and Number of TNF-αin “Zusanli”(ST 36)in C 57 BL/6 J and TRPV1^(-/-)Mice

作　　者：谢璐霜[1,2] 赵娜 杨惠[1] 黄彪[1] 唐勇[1,2] 吴巧凤[1,2] 景向红 余曙光[1,2] XIE Lu-shuang1,2,ZHAO Na1,YANG Hui1,HUANG Biao1,TANG Yong1,2,WU Qiao-feng1,2,JING Xiang-hong3,YU Shu-guang1,2(1 College of Acupuncture and Moxibustion, 2Institute of Acupuncture-moxibustion and Homeostasis Regula tion, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China; 3 Institute of Acupuncture and Moxi bustion , China Academy of Chinese Medical Sciences, Beijing 10070)

机构地区：[1]成都中医药大学针灸推拿学院,成都610075 [2]成都中医药大学针灸与内稳态调节研究所,成都610075 [3]中国中医科学院针灸研究所,北京100700

出　　处：《针刺研究》2018年第5期296-301,共6页Acupuncture Research

基　　金：国家自然科学基金项目(No.81330087;81704160);国家重点基础研究"973"计划项目(No.2015CB 554504);中国博士后科学基金(No.2017 M612928);四川省科技厅课题(No.18YYJC 0999);四川省教育厅课题(No.18TD 0018)

摘　　要：目的:以肿瘤坏死因子-α(TNF-α)为指标,观察瞬时受体电位香草酸亚型1(TRPV 1)是否参与了艾灸启动穴位局部免疫炎性反应的过程。方法:将C 57BL/6J和TRPV1^(-/-)小鼠分别随机分为空白组、30min艾灸组、72h艾灸组,每组5只。各艾灸组均艾灸"足三里",艾灸温度控制在(46±1)℃,通过HE染色观察局部皮肤的形态结构变化,免疫荧光法观察局部皮肤TNF-α阳性细胞的数量。结果:与C 57BL/6J空白组小鼠比较,30min艾灸组表皮TNF-α阳性细胞数明显增多(P<0.05),30min艾灸组、72h艾灸组真皮TNF-α阳性细胞数明显增多(P<0.01);30 min艾灸组真皮TNF-α阳性细胞数明显多于72h艾灸组(P<0.01)。与TRPV1^(-/-)空白组小鼠比较,30min艾灸组表皮TNF-α阳性细胞数明显增多(P<0.01),30min艾灸组、72h艾灸组真皮TNF-α阳性细胞数明显增高(P<0.01);30 min艾灸组小鼠表皮、真皮TNF-α阳性细胞数明显高于72h艾灸组(P<0.01)。结论:艾灸启动穴位局部免疫炎性反应可能不依赖于TRPV 1通道。Objective To observe whether the transient receptor potential vanilloid 1（TRPV 1）plays a role in moxibustion（Moxi）-induced regulation of local immune inflammatory response.Methods Fifteen C 57 BL/6 Jand 15 TRPV1^-/-mice were randomly and respectively divided into blank control,Moxi-30 min and Moxi-72 hgroups（5 mice/group）.The Moxi intervention（[46±1]℃）was applied to the left＂Zusanli＂（ST 36）for 30 min.The local tissue of the left ST 36 region was collected for observing the histological changes after H.E.staining,and for counting the number of tumor necrosis factor-α（TNF-α）immunereaction（IR）-positive cells after immunofluorescence staining.Results In both C 57 BL/6 Jand TRPV 1～（-/-）mice,following Moxi,the local epidermis tissue was incomplete,with vague layers and arrangement of the stratum corneum（being thicker than control mice）,and visible scar tissue.The corium layer was relatively looser in the structure,and the collagenous and elastic fibers were loosened in the arrangement or absence,with an abundant inflammatory cell infiltration,vascular dilation and congestion of blood vessels,and abnormal hyperplasia of hair follicles and sebaceous glands（but without marked structural changes of the subcutaneous tissue）.In C 57 BL/6 Jmice,compared with the control group,the numbers of TNF-αIR-positive cells were significantly increased in the epidermis of the Moxi-30 min group（P〈0.05）,and in the dermis of the Moxi-30 min and-72 hgroups（P〈0.01）.In TRPV1^-/-mice,compared with the control group,the numbers of TNF-αIR-positive cells were also considerably increased in the epidermis of the Moxi-30 min group,and in the dermis of both Moxi-30 min and-72 hgroups（P〈0.01）.Comparison between the two Moxi groups showed that the number of TNF-αpositive cells was significantly lower in the dermis of the Moxi-72 hgroup than in that of the Moxi-30 min group in C 57 BL/6 Jmice（P〈0.01）,and significantly lower in both the epidermis and dermis of the Moxi-72 hgro

关 键 词：艾灸 足三里 皮肤结构变化 肿瘤坏死因子-Α 瞬时受体电位香草酸亚型1 

分 类 号：R245.8[医药卫生—针灸推拿学]