微柱凝胶技术检测血型抗体IgG亚型试验方法的建立  

Establishment of microcolumn gel technology in detection blood type antibody IgG subsets

作　　者：马印图[1] 王更银[1] 李玉秋[2] 李丽君[1] 刘芳[1] 谷建芳[1] 李莉华[2] MA Yintu;WANG Gengyin;LI Yuqiu;LI Lijun;LIU Fang;GU Jianfang;LI Lihua(Department of Blood Transfusion, Bethune International Peace Hospital of PLA, Shijiazhuang, 050082, China;Department of Blood Screening, Hebei Blood Center)

机构地区：[1]中国人民解放军白求恩国际和平医院,石家庄050082 [2]河北省血液中心检验科

出　　处：《临床血液学杂志（输血与检验）》2018年第2期253-256,共4页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

基　　金：河北省中医药局科研计划课题(No:2014068)

摘　　要：目的:建立微柱凝胶技术用于检测血型抗体IgG亚型(IgG_1、IgG_2、IgG_3、IgG_4)的试验方法。方法:筛选6种不同规格的葡聚糖凝胶(颗粒直径在25~100μm)制备微柱凝胶卡,对添加的IgG亚型单抗的最佳稀释度、特异性、重复性和灵敏度进行对比试验,采用正交试验对试剂红细胞浓度及加样量进行筛选。结果:通过筛选抗球蛋白IgG亚类单克隆抗体的效价、亲和力以及与凝胶微粒最佳混合比例,建立凝胶微柱反应体系。选择颗粒直径为75μm(G-75)的葡聚糖凝胶作为灌注凝胶,添加A厂家的IgG亚型单抗4倍稀释后与凝胶混合能够满足检测特异性和灵敏度的要求,加样量选择25μl被检血清与50μl浓度0.8%~1.0%的悬浮红细胞反应,试验结果满意。结论:通过对试验体系的优化组合,成功研制了用于检测血型抗体IgG亚型的微柱凝胶检测技术方法。Objective：To establish the methods of microcolum gel technology in detection the blood type antibody IgG subsets（IgG1,IgG2,IgG3,IgG4）.Method：Preparation by screening 6 different specifications glucan gel（particle diameter from 25 to 100μm）to constitute the microcolum,the best dilution,specificity,repetitive and sensitivity contrast tests were carried out for the antibody IgG subsets.The concentration of red blood cells and volume were screened by orthogonal experiment.Result：The reaction system were established through testing the antibody IgG subsets titer,the appetency and the best gel atom mix ratio.The 75μm particle diameter（G-75）glucan gel and the A vender antibody IgG subsets were selected,The antibody after 4 times diluted mixed with the G-75 glucan to pour into the gel column.The testing results were satisfied selecting reaction sample include 25μl serum and 50μl 0.8%-1.0%red blood solution.Conclusion：A successful method of microcolum gel technology in detection the blood type antibodies IgG subsets was established through the optimization combination of the experimental system.

关 键 词：微柱凝胶技术 血型抗体 IGG亚型 新生儿溶血病 检测方法 

分 类 号：R556[医药卫生—血液循环系统疾病]