Ckip-1对小鼠耳廓软骨发育的影响  

The effects of Ckip-1 on the development of auricular cartilage in mice

作　　者：刘富伟 胡奥 张浚睿 郝英 许珊珊 侯燕 靳丹 赵芝鹤 薄斌 孔亮 LIU Fuwei;HU Ao;ZHANG Junrui;HAO Ying;XU Shanshan;HOU Yan;JIN Dan;ZHAO Zhihe;BO Bin;KONG Liang(710032 Xi＇an, State Key Laboratory of Military Stomatology ＆ National Clinical Research Center for Oral Disease ＆ Shaanxi Key Laboratory of Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology of the Fourth Military Medical University, China)

机构地区：[1]军事口腔医学国家重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病临床医学研究中心,第四军医大学口腔医院口腔颌面外科,西安710032

出　　处：《实用口腔医学杂志》2018年第3期293-297,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金面上项目(编号：81670803)

摘　　要：目的:探讨酪蛋白激酶2相互作用蛋白1(Ckip-1)对小鼠颅颌面耳廓软骨发育的影响。方法:取6月龄Ckip-1基因敲除小鼠(Ckip-1-/-)作为实验组,野生型小鼠(WT)作为对照组(n=6、同窝、雄性)行动物表型观察及组织学切片分析,评估Ckip-1-/-小鼠耳廓大体及组织学变化。另通过siRNA干扰技术下调小鼠软骨细胞系ATDC5中Ckip-1表达,通过扫描电镜、MTT及QPCR等实验,观察细胞形态、增殖能力及软骨细胞分化相关基因表达的变化。结果:Ckip-1-/-小鼠呈小耳畸形,组织学切片可见软骨结构紊乱,幼稚软骨细胞增多。Ckip-1敲低组软骨细胞体现出伸展面积增加、增殖能力下降、软骨早期分化因子增加及晚期分化因子下调等趋势。结论:Ckip-1基因可能通过调控软骨细胞增殖及分化对小鼠耳廓发育产生影响。Objective： To investigate the effects of Casein kinase 2 interacting protein-1 （Ckip-1） on the development of auricular cartilage in mice. Methods： 6-month-old mice with Ckip-1 knockout（ Ckip-1^-/- ） were used as the experimental group, wild type mice（WT） as the control group（n = 6, same litter and gender, male）. The anatomical and histological feature of auricular cartilage was studied by gross observation and histological examination. Ckip-1 was down-regulated in ATDC5 chondrocytes by siRNA interference technique. Scanning electron microscope, MTT and QPCR assays were used to investigate the cell morphology, proliferation, and chondrocyte differentiation related gene expression. Results： Ckip-1^-/- mice showed auricular cartilage deformities and structure disorder, more immature chondrocytes in the cartilge. Down-regulation of Ckip-1 in ATDC5 chondrocytes increased the extensional area of the cells and the expression of early ehondrocyte differentiation factors, the cell proliferation ability and the late differentiation factors were decreased. Conclusion： Ckip-1 may affect auricular development in mice via regulating the proliferation and differentiation of ehondrocytes.

关 键 词：酪蛋白激酶2相互作用蛋白1(Ckip-1) 耳廓 软骨细胞 增殖 分化 

分 类 号：Q78[生物学—分子生物学]