基于转录组测序的慈竹笋木质素基因筛选及其表达分析  

Transcriptome-wide Identification of Lignin Gene Families of Bambusa emeiensis Shoots and Their Differential Expression

作　　者：蒋海港 王树刚 刘阳 沈彦合 李祥宁 曹颖[1] JIANG Hai-Gang;WANG Shu-Gang;LIU Yang;SHEN Yan-He;LI Xiang-Ning;CAO Ying(School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010)

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621010

出　　处：《植物研究》2018年第3期415-421,共7页Bulletin of Botanical Research

基　　金：四川省大学生创新创业训练计划资助项目(16cxcy13);西南科技大学实验技术研究项目(16syjs19);西南科技大学生命科学基地班创新实践项目(SMJDB009)~~

摘　　要：慈竹(Bambusa emeiensis)纤维素含量丰富,是较好的造纸原料,但竹茎中木质素影响着制浆生产及纸浆质量。目前,对慈竹木质素生物合成机制所知甚少,这限制了遗传调控竹木质素的研究。本文以拟南芥、水稻等植物的已知木质素基因作为查询序列,通过BLASTp和系统进化分析,从10、50、100和150 cm慈竹笋转录组数据中筛选到351个木质素生物合成相关Unigenes,包括51个LAC,37个4CL、26个PAL、34个CCR和25个CAD相关转录子,其数量高于其他已报道的竹类植物。转录丰度和定量基因表达分析发现16个木质素基因,包括2个PAL、5个CCR、3个4CL、2个CADH2和4个LAC,随着笋发育而表达上调,表明其可能与发育性木质素积累相关。 Bambusa emeiensis is a good fiber raw material,but the presence of lignin negatively affects bamboo pulp production.The 351 lignin biosynthesis-related unigenes of B.emeiensis were indentified through BLASTp combined with phylogenetic analysis,based on the transcriptome data of B.emeiensis shoots with 10 cm 150 cm height.Among them,the numbers of transcripts including 51 laccase （LAC）,374CL,26PAL and 25CAD were found,and all these transcripts having more abundant in B.emeiensis than other reported bamboo species.RNA-seq and real-time quantitative PCR（RT-qPCR） analysis were used to investigate the differential expression of these lignin genes in bamboo shoots at the different elongation stages,and the relationship between lignin gene expression and lignin amount was further determined.The results revealed that with the elongation of shoots,the expression levels of 16 lignin biosynthesis genes,including 2 PAL,5 CCR,34CL,2CAD and 4LAC,were up-regulated,indicating their roles in developmental lignin accumulation of B.emeiensis.

关 键 词：慈竹 笋 木质素生物合成 差异表达 

分 类 号：S795.5[农业科学—林木遗传育种]