系统性硬化症患者血清IL-33的表达及与TGF-β的相关性  

Expression of IL-33 in patients with systemic sclerosis and its correlation with TGF-β

作　　者：苏斌涛[1] 赵岚[1] 吴行飞[1] 陈馨[1] 陈尊义[2] 殷波涛 SU Bintao;ZHAO Lan;WU Xingfei;CHEN Xin;CHEN Zunyi;YIN Botao(a. Department of Clinical Laboratory, b. Department of Dermatology Laboratory, Wuhan First Hospital, Wuhan 430033, Hubei;Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Hnazhong University of Science and Technology, Wuhan 430030, Hubei, China)

机构地区：[1]武汉市第一医院检验科,武汉430030 [2]武汉市第一医院皮肤病学实验室,武汉430033 [3]华中科技大学同济医学院附属同济医院检验科,武汉430030

出　　处：《临床检验杂志》2018年第4期267-269,共3页Chinese Journal of Clinical Laboratory Science

基　　金：武汉市卫生计生科研项目(WX16D32)

摘　　要：目的探讨白介素33(IL-33)、可溶性生长刺激表达基因2蛋白(s ST2)及转录生长因子1(TGF-β1)在系统性硬化症患者中的表达及其相关性。方法应用ELISA检测25例系统性硬化症患者、25例体检健康者血清中IL-33、s ST2以及TGF-β1的表达,采用免疫组织化学技术检测IL-33在系统性硬化症患者皮损和正常皮肤组织中的表达差异。结果与健康人对照组相比,系统性硬化症患者血清中IL-33及TGF-β1的表达水平显著升高(P<0.05),且系统性硬化症患者中IL-33浓度与TGF-β1的表达水平呈正相关(P<0.05);免疫组织化学结果提示IL-33在系统性硬化症患者的皮损组织中表达增多;而s ST2的表达水平在系统性硬化症患者及健康人对照组中差异无统计学意义。结论系统性硬化症患者外周血IL-33浓度升高,与TGF-β1的表达相关,提示IL-33可能通过TGF-β1的调控,在炎症和纤维化过程中发挥重要作用。Objective To investigate the expressions of interleukin-33 （IL-33）, soluble growth stimulation expressed gene 2 （sST2） and transforming growth factor β1 （TGF-β1） in the patients with systemic sclerosis （SSc） and their correlations. Methods Serum IL-33, sST2 and TGF-β1 levels from 25 SSc patients and 25 healthy controls were determined by an enzyme linked immunosorbent assay (ELISA）. The expressions of IL-33 in the damaged skin tissue of SSc patients and normal skin tissue were detected with the immunohistochemical staining. Results Serum IL-33 and TGF-β1 levels in SSc patients were significantly higher than that in healthy controis （P 〈 0.05）, and serum IL-33 levels were positively correlated with TGF-β1 levels in SSc patients (P 〈 0.05). Immunohistochemical staining results showed that the expression level of IL-33 in the damaged skin tissue of SSc patients were significantly higher than that in normal skin tissue （P 〈 0.05). However, there was no significant difference in the expression level of sST2 between SSc patinets and healthy controls. Conclusion Serum IL-33 levels in SSe patients increase significantly, which is correlated with the expression of TGF-β1, indicating that IL-33 may play an important role in the process of inflammation and fibrosis of SSc via the regulation of TGF-β1.

关 键 词：系统性硬化症 白介素33 生长刺激表达基因2蛋白 转录生长因子 

分 类 号：R446.6[医药卫生—诊断学]