电感耦合等离子体发射光谱法测定人血浆中5种金属元素  

Simultaneous Determination of Five Metals in Human Plasma by Inductively Coupled Plasma Optical Emission Spectrometry

作　　者：武鑫 赵玮[1] 宋楠楠 闫慧芳[1] WU Xin1;ZHAO Wei1;SONG Nannan2;YAN Huifang1

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所 [2]首都经济贸易大学

出　　处：《环境卫生学杂志》2018年第2期134-138,共5页JOURNAL OF ENVIRONMENTAL HYGIENE

基　　金：国家科技支撑计划课题(2014BAI12B00;2006BAI06B00)

摘　　要：目的建立人血浆中锌、铁、镁、钙、铜5种金属元素的电感耦合等离子体发射光谱(ICP-OES)测定方法。方法采用1%(v/v)硝酸对血浆进行10倍稀释后直接进行ICP-OES分析,标准曲线法定量。结果 5种金属元素在对应的浓度范围内线性良好,相关系数均>0.999,方法检出限为(0.004 1~0.015 1)mg/L。方法批内精密度(RSD%)为0.8%~2.5%,方法批间精密度(RSD%)为1.4%~3.0%,平均加标回收率范围为93.3%~105.2%;血清标准物质中5种元素测定结果均在参考值范围内。结论建立的方法可用于人血浆中5种金属元素的测定。Objectives To establish an inductively coupled plasma optical emission spectrometry(ICP-OES）for the determination of 5 metals,including Zn,Fe,Mg,Ca and Cu in human plasma.Methods Plasma samples were analyzed by ICP-OES after 10 times dilution with 1%(v/v） nitric acid solution and quantified by standard curve method.Results There were good linearity of standard curves in the detection range of(0.004 1-0.015 1） mg/L for 5 metals,with a correlation coefficient of 0.999.The intra-assay precision of 5 metals were 0.8%-2.5% and the inter-assay precision of 5 metal were 1.4%-3.0%.The spiked for all elements was in the range of 93.3%-105.2%.The result of five elements in the reference materials were in the acceptable ranges.Conclusions The inductively coupled plasma optical emission spectrometry(ICP-OES） method could be used to monitor the concentrations of the 5 metals in human plasma.

关 键 词：人血浆 金属元素 电感耦合等离子体发射光谱法 

分 类 号：R126.8[医药卫生—环境卫生学]