UPLC-MS/MS检测中药及保健品中添加的9种改善胃肠功能类化学药物  

Determination of 9 Supplementary Chemical Drugs in Traditional Chinese Medicines and Health Care Products for Improving Gastrointestinal Function by UPLC-MS/MS

作　　者：李锐[1] 张海防[1] LI Rui;ZHANG Hal-fang(Hainan Provincial Institute for Drug Control, Haikou Hainan 570216, China)

机构地区：[1]海南省药品检验所,海南海口570216

出　　处：《中医药导报》2018年第10期96-99,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:建立同时测定中药及保健品中添加的9种改善肠胃功能类化学药物的超高效液相色谱-串联质谱(UPLC-MS/MS)法。方法:采用Agilent ZORBAX SB-C18(2.1 mm×50 mm,1.8μm)色谱柱,以乙腈-0.1%甲酸溶液(22∶78)为流动相,流速为0.2 m L/min。质谱采用电喷雾离子源(ESI)正离子检测,以多重反应监测(MRM)方式进行定量。结果:9种药物在10~1,000 ng/m L范围内呈现良好的线性关系,r>0.998。9种药物在10 ng/m L添加水平的加样回收率93.6%~102.4%,RSD%<5%。15批样品中检出3批阳性样品。结论:该方法可作为中药及保健品中非法添加西咪替丁、雷尼替丁、法莫替丁、奥美拉唑、泮托拉唑、兰索拉唑、多潘立酮、莫沙必利及西沙必利等9种化学药物的检测方法。Objective： To establish a UPLC-MS/MS method for the determination of 9 supplementary chemical drugs traditional Chinese medicines and health care products for improving gastrointestinal function. Methods：The analysis was performed on a Agilent ZORBAX SB-C18 column（2.1 mm×50 mm, 1.8 μm）. Mobile phase was acetonitrile-0.1% formic acid water solution（22 ∶78） at a flow rate of 0.2 m L/min. Electrospray ionization（ESI）source was applied in the positive mode. Multiple reaction monitoring（MRM） mode was used to quantify 9 chemical drugs. Results： The correlation coefficients of 9 drugs were all more than 0.998 with linear range 10-1,000 ng/m L. The average recovery of 9 drugs at the spiked level of 10 ng/m L was between 93.6%-102.4% with the relative standard deviation less than 5%. Three positive samples in 15 batchs samples were detected. Conclusion： The method can be used to determin 9 chemical drugs, including cimitidine, ranitidine, famotidine,omeprazole, pantoprazole, lansoprazole, domperidone, mosapride and cisapride.

关 键 词：中药 保健品 西咪替丁 雷尼替丁 法莫替丁 奥美拉唑 泮托拉唑 兰索拉唑 多潘立酮 莫沙必利 西沙必利 高效液相色谱-串联质谱 非法添加 

分 类 号：R284[医药卫生—中药学]