右美托咪定对内毒素性休克大鼠海马及齿状回c-fos表达的影响  

Effects of dexmedetomidine on expression of c-fos in hippocampus and dentate gyrus in a rat modelof endotoxic shock

作　　者：熊波 缪长虹 Xiong Bo;Miao Changhong(Department of Anesthesiology, Cancer Hospital, Fudan University, Shanghai 200032, Chin)

机构地区：[1]复旦大学附属肿瘤医院麻醉科,上海市200032

出　　处：《中华麻醉学杂志》2018年第2期223-225,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（201181171836）

摘　　要：目的评价右美托咪定对内毒素性休克大鼠海马和齿状回c-fos表达的影响。方法清洁级健康雄性sD大鼠35只，3-4月龄，体重250-300g。采用随机数字表法分为5组（n=7）：生理盐水组（Ns组）、右美托咪定组（D组）、内毒素性休克组（Es组）、低剂量右美托咪定＋LPS组（LD组）和高剂量右美托咪定＋LPS组（HD组）。D组和LD组分别尾静脉注射右美托咪定0．5μg／kg，HD组注射右美托咪定4．5μg／kg。NS组和Es组注射生理盐水0．5ml／kg，5min后，Ns组和D组注射生理盐水0．5ml／kg，其余组注射LPS5mg／kg，注射时间均为10min。注射LPS后6h时处死大鼠取脑组织，分离海马和齿状回，采用免疫组化法检测c-fos的表达。结果与Ns组或D组比较，Es组海马和齿状回c-fos表达上调（P〈0．05）；与LPS组比较，LD组和HD组海马和齿状回c-fos表达下调（P〈0．05）；与LD组比较，HD组海马CAl和CA3区c-fos表达下调（P〈0．05）。结论右美托咪定产生脑保护作用的机制与抑制内毒素性休克大鼠海马和齿状回c-fos表达上调有关。Objective To evaluate the effects of dexmedetomidine on the expression of c-fos in hip-pocampus and dentate gyrus in a rat model of endotoxic shock. Methods Thirty-five pathogen-free healthy male Sprague-Dawley rats, aged 3-4 months, weighing 250-300 g, were divided into 5 groups (n = 7 each） using a random number table： normal saline group （group NS）, dexmedetomidine group （group D） , endotoxic shock group （group ES） , low-dose dexmedetomidine plus lipopolysaceharide （LPS） group (group LD） and high-dose dexmedetomidine plus LPS group (group HD）. Dexmedetomidine 0. 5 p,g/kg was injected via the tail vein in D and LD groups, and dexmedetomidine 4. 5 p,g/kg was given in group HD. Normal saline 0. 5 ml/kg was injected in NS and ES groups, 5 min later normal saline 0. 5 ml/kg was injec-ted in NS and D groups and LPS 5 mg/kg was injected in the other groups, and the injection time was 10 min in all groups. Rats were sacrificed at 6 h after LPS injection, brains were removed, and the hippocam-pus and dentate gyrus were isolated for detection of the expression of c-los by immunohistochemistry. Re-sults Compared with group NS or group D, the expression of c-los in the hippocampus and dentate gyrus was significantly up-regulated in group ES （P〈0. 05）. Compared with group LPS, the expression of c-fos in the hippocampus and dentate gyrus was significantly down-regulated in LD and HD groups （P〈0. 05）. Compared with group LD, the expression of c-fos in hippocampal CA1 and CA3 areas was significantly down-regnlated in group HD （P〈0. 05）. Conclusion The neuroprotective mechanism of dexmedetomi-dine is related to inhibiting the up-regulated expression of c-fos in the hippocampus and dentate gyrus in a rat model of endotoxic shock.

关 键 词：右美托咪啶 内毒素血症 海马 齿状回 原癌基因蛋白质C-FOS 

分 类 号：R614[医药卫生—麻醉学]