非梗阻性无精子症和严重少精子症患者微核率的观察  

Observation of micronucleus rate in patients with non-obstructive azoospermia and severe oligozoospermia

作　　者：蔡文侠 吴文彬 林丰 钱定良 CAI Wen-xia;WU Wen-bin;LIN Feng;QIAN Ding-liang.(Third Affiliated Hospital of Wenzhou Medical University, Ruian 325200, Chin)

机构地区：[1]温州医科大学附属第三医院,浙江瑞安325200

出　　处：《中国优生与遗传杂志》2018年第5期117-118,86,共3页Chinese Journal of Birth Health & Heredity

基　　金：温州市科技项目(Y20130310)子项目

摘　　要：目的探讨外周血淋巴细胞微核率与非梗阻性无精子症(A组)和严重少精子症(B组)形成的相关性,为进行病因学研究和采取干预措施提供理论依据。方法分别对38例A组和32例B组患者及30例已经生育的男性,按外周血淋巴细胞微核试验方法进行培养检测,分别计数微核,计算微核细胞率和微核率。结果微核细胞率、微核率分别为:实验A组5.19±1.58‰、6.37±1.63‰,B组4.51±1.55‰、5.94±1.61‰;对照组为1.87±1.63‰、2.27±1.72‰,实验A、B组和对照组对比差异皆有统计学意义(P<0.01),实验A、B组之间差异无统计学意义(P>0.05)。结论非梗阻性无精子症和严重少精子症患者外周血淋巴细胞微核率明显增高,表明两者有遗传损伤即具染色体不稳定性。Objective：To investigate the correlation between the micronucleus rate of peripheral blood lymphocytes and the formation of non-obstructive azoospermia（group A）and severe oligospermia（group B）,and to provide a theoretical basis for the etiological study and intervention. Methods：38 cases of A and 32 cases of group B patients and 30 cases of male fertility were detected by peripheral blood lymphocyte micronucleus test method were detected micronuclei were counted and the rate of micronuclei and micronucleus were calculated. Results：The rates of micronuclei and micronuclei were 5.19±1.58 ‰ and 6.37±1.63‰ in group A,4.51±1.55‰ and 5.94±1.61‰ in group B,and 1.87±1.63 ‰ and 2.27±1.72 ‰ in group B,There was significant difference between experimental group A and B and control group（P〈0.01）. There was no significant difference between experimental group A and group B（P〉0.05）. Conclusion：Non-obstructive azoospermia and severe oligospermia in peripheral blood lymphocytes micronucleus rate was significantly increased,indicating that both have genetic damage that is chromosomal instability.

关 键 词：非梗阻性无精子症 严重少精子症 微核率 染色体不稳定性 

分 类 号：R698.2[医药卫生—泌尿科学]