犀角地黄汤合银翘散对流感病毒性肺炎小鼠肺组织GRKs-βAR-Gsα信号通路的影响  

Impacts of the Combined Medication of Xijiao Dihuang Tang and Yinqiao San on GRKs-βAR-Gsα Signaling Pathways in the Lung Tissues of Mice with Influenza Viral Pneumonia

作　　者：赵琦 席榕 张淑静 齐晓宇 陈晓鸣 张俊杰 郑建铖 朱名扬 李础含 吴莹 ZHAO Qi;XI Rong;ZHANG Shu - jing;QI Xiao - yu;CHEN Xiao - ruing;ZHANG Jun - jie;ZHENG Jian - cheng;ZHU Ming- yang;LI Chu- han;WU Ying(School of Life Science, Beijing University of Traditional Chinese Medicine, Beijing 100029)

机构地区：[1]北京中医药大学生命科学学院,北京100029

出　　处：《世界中西医结合杂志》2018年第4期487-491,496,共6页World Journal of Integrated Traditional and Western Medicine

基　　金：2016年北京市共建项目(BJGJ1610)

摘　　要：目的以流感病毒性肺炎小鼠作为模型,检测犀角地黄汤合银翘散(XDY)对小鼠肺组织中GRKs-βAR-Gsα通路的影响,以明确其改善肺血管通透性的分子机制。方法将50只雄性Balb/C小鼠按体重随机分为5组,每组10只,分别为正常组、病毒组和犀角地黄汤高、中、低剂量组。每组除正常组外,均以A/FM/1/34(H1N1)病毒株滴鼻感染。1 h后,正常组、病毒组用蒸馏水灌胃,2次/d;其余三组分别用高、中、低剂量的XDY灌胃,2次/d。在感染后的第3天、第5天分别摘眼球处死小鼠,荧光定量PCR检测各组小鼠肺组织β-AR mRNA含量;Western blot检测肺组织GRK2、Gsα的蛋白表达水平。结果第3天:与病毒组相比,XDY中剂量组GRK2蛋白表达水平显著下降(P<0.001),β-AR含量升高(P<0.01),而Gsα的表达无明显差异(P>0.05);第5天:与病毒组相比,XDY中剂量组GRK2蛋白表达水平仍显著下降(P<0.01),β-AR含量显著升高(P<0.001),Gsα的表达明显升高(P<0.05),差异有统计学意义。结论流感病毒感染后,犀角地黄汤合银翘散可通过抑制GRK2蛋白表达,升高β-AR、Gsα含量,改善流感病毒肺炎小鼠肺血管通透性,从而发挥抗炎作用。Objective To observe the impacts of the combined medication of xijiao dihuang tang and yinqiao san(XDY） on GRKs-βAR-GSα signaling pathways in the lung tissues of mice with influenza viral pneumonia,taking the mice of influenza viral pneumonia as models,so as to determine the molecular mechanism on the improvements of pulmonary vascular permeability. Methods A total of 50 male Balb/C mice were randomized into 5 groups according to the body weight,10 mice in each one,including a normal group,a virus group and XDY high-dose,middle-dose and low-dose groups. Except the normal group,the mice in the rest groups were infected with A/FM/1/34/(H1 N1） via nasal drop. 1 h later,the distilled water was used for lavage in the normal and virus groups,twice a day. XDY of high,middle and low doses were used for lavage in the rest groups separately,twice a day. On day3 and day5 after infection,the mice were sacrificed and the eyeballs extracted. The real time fluorogenic quantitative PCR was used to determine the level ofβ-AR mRNA in lung tissue. Western blot was used to determine the protein expression of GRK2 and GSα.Results On day3,compared with the virus group,the GRK2 protein expression was reduced significantly(P 〈0. 001）,β-AR was increased(P 〈 0. 01） and GSα did not change(P 〉0. 05） in the XDY middle-dose group. On day5,compared with the virus group,the GRK2 protein expression was reduced significantly(P 〈0. 01）,β-AR was increased significantly(P 〈 0. 001） and GSα was increased remarkably(P 〈 0. 05） in the XDY middle-dose group,indicating the significant differences. Conclusion After influenza viral infection,the combined medication of XDY plays its role of anti-inflammation through inhibiting GRK2 protein expression,increasing the levels of β-AR and GSα and improving the pulmonary vascular permeability of mice with influenza viral Pneumonia.

关 键 词：犀角地黄汤合银翘散 流感病毒 荧光定量PCR WESTERN BLOT GRKs Β-AR Gsα 

分 类 号：R285.5[医药卫生—中药学]