猪胸膜肺炎放线杆菌的遗传演化关系分析  

Genetic evolution analysis of Actinobacillus pleuropneumoniae

作　　者：梁昱 刘燕霏 杨建德 LIANG Yu;LIU Yanfei;YANG Jiande(College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, Chin)

机构地区：[1]天津农学院动物科学与动物医学学院

出　　处：《黑龙江畜牧兽医》2018年第9期103-105,110,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：教育部留学回国基金项目;天津自然科学项目(07JCYBJC16000);天津农学院附属动物医院关键技术集成与学生综合能力提升项目(ZH004901)

摘　　要：为了探究不同国家猪胸膜肺炎放线杆菌（Actinobacillus pleuropneumoniae,App）的遗传演化规律,明确不同地区App的亲缘关系,试验采用生物信息学方法,对NCBI中App的16S rRNA基因进行整理,剪裁对齐后构建基因进化树并进行分析比较。结果表明：App在进化过程中呈现区域性稳定遗传现象,源自中国的分离株聚为一支,呈现高度的保守性,并与英国、美国App菌株有一定亲缘关系,而与周边日本菌株关系较远;亚欧大陆上不同国家的菌株同源性较高,两地区的分离株在进化树上起源于共同的祖先。In order to explore the genetic evolution of Actinobacillus pleuropneumoniae（App） in different countries and clarify the relationships among different App in each regions,16 S rRNA gene of App in the NCBI nucleotide database were analyzed and compared by the bioinformatics method. The phylogenetic tree was constructed after tailoring alignment. The results showed that a stable genetic phenomenon was indicated in the evolutionary process of App. The isolates derived from China were clustered and showed a high degree of conservation. They had a certain genetic relationship with the British and American strains,but had far relationship with the Japan which was neighboring country of china. The isolates from different countries in the Eurasian continent had high homology. The isolates of the two regions originated from the common ancestors.

关 键 词：猪胸膜肺炎放线杆菌 遗传演化 进化树 同源性比较 来源地 亲缘关系 

分 类 号：S852.619[农业科学—基础兽医学] S858.28[农业科学—兽医学]