藏茵陈4种有效成分的含量测定  

Determination of four active ingredients of Zangyinchen

作　　者：张慧[1] 杨孟妮 李洋[1] 刘娟[1] 李文婷 夏厚林[1] ZHANG Hui;YANG Meng-ni;LI Yang;LIU Juan;LI Wen-ting;XIA Hou-lin(School of Pharmacy, Chengdu University of Traditional Chinese Medicine,＂ Key Laboratory of Standardization for Chinese Herbal Medicine, Ministry of Educatio;National Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu 61113 7, Sichua)

机构地区：[1]成都中医药大学、中药材标准化教育部重点实验室、四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地

出　　处：《中药与临床》2017年第4期10-13,共4页Pharmacy and Clinics of Chinese Materia Medica

摘　　要：目的:建立藏茵陈4种有效成分的含量测定方法,并测定10批不同来源的含量。方法:采用HPLC(高效液相色谱法)法,同时测定藏茵陈中獐牙菜苦苷、龙胆苦苷、獐牙菜苷和芒果苷的含量,采用C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.1%磷酸水溶液梯度洗脱,检测波长238 nm(獐牙菜苦苷)、274 nm(龙胆苦苷)、246 nm(獐牙菜苷)和258nm(芒果苷)。结果:建立了HPLC法同时测定藏茵陈中四种有效成分含量的方法,四种成分分离度良好,方法学考察符合要求。结论:本方法操作简便,结果准确,重复性好,可靠性好,可用于同时测定藏茵陈中四种有效成分的含量。Objective：To establish determination method of four effective components in Zangyinchen,and detect the content 5 of 10 batches samples from different sources.Method：High performance liquid chromatography（HPLC） method was used for the simultaneous determination of swertiamarin,gentiopicroside,swertiamarin and mangiferin in Zangyinchen on C18 column（4.6 mm × 250 mm,5 μm）,with methanol-0.1% phosphoric acid water solution as mobile phase in gradient elution.The detection wavelength was set at 238 nm（swertiamarin）,274 nm（gentiopicroside）,243 nm（sweroside） and 258 nm（mangiferin）.Result：The HPLC simultaneous determination of four effective components in Zangyinchen was established with good separation,and met the methodological requirements.Conclusion：The method is simple,accurate with ood repeatability and reliability,which can be used for the simultaneous determination of four effective components in Zangyinchen.

关 键 词：藏茵陈 有效成分 HPLC 含量测定 

分 类 号：R284.1[医药卫生—中药学]