歼击机飞行员外周血淋巴细胞及亚群的分析  

A preliminary study of lymphocyte and its subsets in peripheral blood of fighter pilots

作　　者：薛梅[1] 朱玲[1] 刘静[1] 韩东梅[1] 郑晓丽[1] 丁丽[1] 朱培玉 王恒湘[1] Xue Mei;Zhu Ling;Liu Jing;Han Dongmei;Zheng Xiaoli;Ding Li;Zhu Peiyu;Wang Hengxiang.(Department of Hematology, Air Force General Hospital, Beijing 100142, China)

机构地区：[1]空军总医院,北京100142

出　　处：《中华航空航天医学杂志》2017年第3期169-173,共5页Chinese Journal of Aerospace Medicine

基　　金：总后面上课题（CWS12J080）

摘　　要：目的 分析航空环境对空军歼击机飞行员外周血淋巴细胞及亚群的影响,为积极采取相应的防护措施,调整飞行员淋巴细胞免疫功能提供数据基础. 方法 采集51例入院体检的空军歼击机飞行员（飞行员组）及25例同期健康体检的男性普通军人（对照组）的外周血,用流式细胞术检测外周血CD45^＋淋巴细胞、CD3＋T淋巴细胞、CD4^＋T淋巴细胞、CD8^＋T淋巴细胞、初始T淋巴细胞、记忆T淋巴细胞及调节性T淋巴细胞的表面标志.飞行员按飞行时间分为飞行时间＜1 000 h、1 000-2 000 h、＞2 000 h 3组.比较飞行员组与对照组间以及不同飞行时间组间各检测指标的差异. 结果 飞行员组外周血CD45^＋淋巴细胞、CD8^＋T淋巴细胞表面标志均值明显高于对照组（0.365±0.074与0.308±0.084和0.370±0.075与0.320±0.071）,差异有统计学意义（t=3.051、2.752,P＜0.01）;CD4^＋/CD8^＋比值低于对照组（1.34±0.54与2.04±1.54）,差异有统计学意义（t=2.928,P＜0.01）;其他指标两组间差异无统计学意义.不同飞行时间组间的外周血CD4^＋/CD8^＋比值均值、初始CD8^＋T淋巴细胞、记忆CD8^＋T淋巴细胞表面标志均值的差异有统计学意义（P＜0.05）,而CD45^＋淋巴细胞、CD3＋T淋巴细胞、CD4^＋T淋巴细胞、CD8^＋T淋巴细胞、初始CD4^＋T淋巴细胞、记忆CD4^＋T淋巴细胞、调节性T淋巴细胞表面标志均值的差异无统计学意义（P＞0.05）. 结论 本组飞行员外周血CD45^＋淋巴细胞、CD8^＋T淋巴细胞表面标志均值及CD4^＋/CD8^＋比值与对照组存在差异;不同飞行时间组飞行员外周血CD4^＋/CD8^＋比值及初始CD8^＋T淋巴细胞、记忆CD8^＋T淋巴细胞表面标志均值存在差异;提示歼击机飞行员的免疫系统有一定变化,可能与飞行环境有关,应积极采取相应的防护措施.Objective To suggest countermeasures by analyzing the aviation effects on pilots＇ lymphocyte and its subsets in peripheral blood and to provide basis for taking active counter measures and managing their immune function of lymphocyte.Methods We sampled the peripheral blood from 51 male fighter pilots （pilot group）,who were hospitalized for physical examination,and 25 healthy male military personnel （control group） to detect the surface markers of CD45^＋ lymphocytes,CD3＋T lymphocytes,CD4^＋T lymphocytes,CD8^＋T lymphocytes,native T lyrnphocytes,memory T lymphocytes and regulatory T lymphocytes in peripheral blood by flow cytometry.The pilot group was further divided into other subgroups by flying hours：〈1 000 h,1 000-2 000 h and 〉2 000 h.The detect results were compared between pilot and control groups and among flying hour groups.Results As compared with the control group,the mean values of surface marker of CD45^＋ lymphocyte and CD8^＋ T lymphocytes in peripheral blood of pilot group were significantly higher （0.365±0.074 vs.0.308±0.084,0.370±0.075 vs.0.320＋0.071）.The comparison showed statistical differences （t=3.051,2.752,P〈0.01）.The CD4^＋/CD8^＋ ratio of pilot group was significantly lower than that of control group （1.34±0.54 vs.2.04±1.54,t=2.928,P〈0.01）.Other comparisons had no significant difference between two groups.The mean value of CD4^＋/CD8^＋ ratio,the mean values of the surface marker of CD8^＋ native T lymphocytes and CD8^＋ memory T lymphocytes in peripheral blood showed significances among different flying hour groups （P〉0.05）,but the mean values of surface marker CD45^＋ lymphocytes,CD3＋ T lymphocytes,CD4^＋ T lymphocytes,CD8^＋ T iymphocytes,CD4^＋ native T lymphocytes,CD4^＋ memory T lymphocytes and regulatory T lymphocytes had no significant difference（P〉0.05）.Conclusions There are statistical differences on the mean values of surface marker of CD45^＋ lymphocytes,CD8^＋ T lymphocytes in peripheral blood and CD4^＋/

关 键 词：CD4阳性T淋巴细胞 CD8阳性T淋巴细胞 淋巴细胞亚群 歼击机飞行员 

分 类 号：R856.9[医药卫生—航空、航天与航海医学] R392.12[医药卫生—临床医学]