高产3-羟基丁酮解淀粉芽孢杆菌的选育及发酵优化  

Mutation and fermentation optimization of Bacillus amyloliquefaciens for acetoin production

作　　者：王诗卉 罗秋玲[1,2,3] 刘佳 刘立明[1,2,3] 陈修来 Shihui Wang1,2,3, Qiuling Luo1,2,3, Jia Liu1,2,3, Liming Liu1,2,3, and Xiulai Chen1,2,3(1 State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China; 2 Key Laboratory of Industrial Bioteehnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China; 3 National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, Jiangsu, Chin)

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [2]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [3]江南大学粮食发酵工艺与技术国家工程实验室,江苏无锡214122

出　　处：《生物工程学报》2018年第5期803-811,共9页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(No.2016YFD0400801);江苏省科技支撑计划社会发展项目(No.BE2017622)资助~~

摘　　要：3-羟基丁酮(Acetoin)作为一种重要的食用香料和平台化合物被广泛应用于医药、食品等领域。为改善解淀粉芽孢杆菌Bacillus amyloliquefaciens的3-羟基丁酮生产能力,采用常压室温等离子体(ARTP)和^(60)Coγ射线进行复合诱变,以3-羟基丁酮产量为分析指标,筛选获得最优突变株B.amyloliquefaciens H-5,3-羟基丁酮产量为68.2 g/L。为进一步实现3-羟基丁酮的高效生产,对此突变株进行5 L发酵罐水平的培养条件优化,并于30 L发酵罐上进行放大培养,最终3-羟基丁酮产量达85.2 g/L,较出发菌株B.amyloliquefaciens FMME088提高了26.8%。上述研究结果表明,ARTP和^(60)Coγ射线复合诱变能够有效获得高产菌株,该突变株具有较高的工业化微生物发酵生产3-羟基丁酮的潜能。As a platform chemical, acetoin has a great potential of application in medicine and food industries. In order to improve the efficiency of acetoin production, Bacillus amyloliquefaciens was treated by atmospheric and room temperature plasma and gamma rays. Two-round screening was adopted for obtaining positive mutants, and the best mutant B. amyloliquefaciens H-5 produced acetoin up to 68.2 g/L in shake flask. Then, culture conditions were optimized in 5-L fermentor to enhance acetoin production. Finally, 85.2 g/L acetoin was produced by B. amyloliquefaciens H-5, which was increased by 26.8% compared with that of the original strain B. amyloliquefaciens FMME088. These results indicated that the high-producing strain can be obtained efficiently by compound mutagenesis, which has a promising prospect for commercial scale process.

关 键 词：解淀粉芽孢杆菌 3-羟基丁酮 常压室温等离子体 ^60Coγ射线 发酵条件优化 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TS264.3